African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Assessment of genetic diversity among different indigenous Xanthomonas isolates via randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR)

Sabin Fatima1, Rukhsana Bajwa1, Tehmina Anjum1*and Zafar Saleem2
1Institute of Agriculture Sciences, Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan. 2Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Email: [email protected]

  •  Accepted: 12 December 2011
  •  Published: 31 March 2012

Abstract

The genetic diversity among seven Xanthomonas isolates representing four species was assessed using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) PCR-based techniques. Both techniques revealed high degrees of polymorphisms among the studied isolates. Cluster dendrogram based on combined data of RAPD and ISSR showed that genetic diversity exists in local isolates of Xanthomonas. In terms of percentage similarity values, the genomic variation was found to be in the range of 29.29 to 100% among the isolates. X. campestris (Mangifera indica) remain unclustered in cluster dendrogram and showed a unique genomic profile as compared to other isolates used in this study.

Key words: Xanthomonas, genetic diversity, randomly amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR).

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This article is published under the terms of the Creative Commons Attribution License 4.0

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