African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Characterization of baculovirus isolates obtained from soil by restriction fragment patterns

Claudio Rios-Velasco1*, David I. Berlanga-Reyes1, Gabriel Gallegos-Morales2 and Ma. Cristina Del Rincón-Castro3
1Centro de Investigación en Alimentación y Desarrollo, AC. Unidad Cuauhtémoc, Chihuahua. Av. Río Conchos S/N Parque Industrial. Apdo. Postal 781. CP. 31570 Cd. Cuauhtémoc, Chihuahua, México. 2Departamento de Parasitología, Universidad Autónoma Agraria Antonio Narro, Calzada Antonio Narro 1923, Buenavista, Saltillo, Coahuila, C.P. 25315. México. 3Departamento de Alimentos, División de Ciencias de la Vida, Campus Irapuato-Salamanca, Universidad de Guanajuato, Ex-Hacienda El Copal, Km. 9 Carretera Irapuato-Silao, C.P. 36500 Irapuato, Guanajuato, México.
Email: [email protected]

  •  Accepted: 23 April 2012
  •  Published: 09 June 2012

Abstract

Four baculovirus isolates recovered from soil in corn plots infested with fall armyworm (FAW) larvae were characterized using three different restriction enzymes. Each isolate wasamplified in larvae in aseptic conditions, to increase the number of occlusion bodies (OBs). Baculovirus isolates were purified on continuous sucrose gradients by ultracentrifugation.The purified DNA of each isolate was digested with restriction enzymes EcoRI, HindIII andBamHI. Each isolate showed a significant difference in the fragments obtained byelectrophoresis. Isolates CAD and NAV1 showed similar band patterns, the AN1 and AN2isolates had different patterns between them and the others. Virulent isolates of baculovirus can be obtained from soil, which can be an important reservoir of these entomopathogenous viruses.

 

Key words: Baculoviridae, nucleopolyhedrovirus, granulovirus, fall armyworm, restriction enzymes.

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