Seroepidemiology, molecular and entomological studies of bluetongue in sheep in Gujarat

Chauhan H. C.; Bhagat A. G.; Dadawala A. I.; Patel B. K.; Chandel B. S.; Kher H. N.; Patel M. A.; Rajgor M.

doi:10.5897/AJMR2016.8002

African Journal of
Microbiology Research

Abbreviation: Afr. J. Microbiol. Res.
Language: English
ISSN: 1996-0808
DOI: 10.5897/AJMR
Start Year: 2007
Published Articles: 5233

Full Length Research Paper

Seroepidemiology, molecular and entomological studies of bluetongue in sheep in Gujarat

Chauhan H. C.

Chauhan H. C.
Department of Animal Biotechnology, College of Veterinary Science and AH, S.D.A.U., Sardarkrushinagar- 385 506 (B.K.), Gujarat, India.
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Bhagat A. G.

Bhagat A. G.
Department of Microbiology, College of Veterinary Science and AH, S.D.A.U., Sardarkrushinagar- 385 506 (B.K.), Gujarat, India.
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Dadawala A. I.

Dadawala A. I.
Department of Microbiology, College of Veterinary Science and AH, S.D.A.U., Sardarkrushinagar- 385 506 (B.K.), Gujarat, India.
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Patel B. K.

Patel B. K.
Department of Animal Biotechnology, College of Veterinary Science and AH, S.D.A.U., Sardarkrushinagar- 385 506 (B.K.), Gujarat, India.
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Chandel B. S.

Chandel B. S.
Department of Animal Biotechnology, College of Veterinary Science and AH, S.D.A.U., Sardarkrushinagar- 385 506 (B.K.), Gujarat, India.
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Kher H. N.

Kher H. N.
Department of Microbiology, College of Veterinary Science and AH, S.D.A.U., Sardarkrushinagar- 385 506 (B.K.), Gujarat, India.
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Patel M. A.

Patel M. A.
Department of Animal Biotechnology, College of Veterinary Science and AH, S.D.A.U., Sardarkrushinagar- 385 506 (B.K.), Gujarat, India.
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Rajgor M.

Rajgor M.
Department of Microbiology, College of Veterinary Science and AH, S.D.A.U., Sardarkrushinagar- 385 506 (B.K.), Gujarat, India.
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Article Number - A9868A459080
Vol.10(23), pp. 856-860 , June 2016
https://doi.org/10.5897/AJMR2016.8002

Received: 10 March 2016
Accepted: 27 May 2016
Published: 21 June 2016

Abstract

Seroepidemiology, molecular and entomological studies of bluetongue in sheep was carried out in Gujarat, India. Out of 980 sera sample screened, 361 (36.84 %) were found positive by c-enzyme-linked immunosorbent assay (c-ELISA). Out of the 103 blood samples tested for the presence of bluetongue virus (BTV) by reverse transcription-polymerase chain reaction (RT-PCR), 3 were found positive, producing 274 bp amplicons with NS1 gene specific primers. Collection and identification of Culicoides vectors from various farms suggest that the Culicoides oxystoma is the most prevalent species.

Key words: Bluetongue, sheep, c-Enzyme-linked Immunosorbent Assay (c-ELISA), NS1 gene, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), Culicoides.