African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Simple and rapid detection of Salmonella sp. from cattle feces using polymerase chain reaction (PCR) in Iran

Aida Jadidi1, Seyed Davood Hosseni2*, Alireza Homayounimehr3, Adel Hamidi2, Sepideh Ghani4 and Behnam Rafiee4
  1Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran. 2Department of Cellular and Molecular Biology, Razi Vaccination and Serum Research Institute, Arak, Iran. 3Department of Medical Science, Arak Branch ,Islamic Azad University, Arak, Iran. 4Young Researchers Club, Arak Branch, Islamic Azad University, Arak, Iran.
Email: [email protected]

  •  Accepted: 21 March 2012
  •  Published: 28 June 2012

Abstract

 

The aim of this study was to employ biochemical and molecular assays to detect and diagnose Salmonella in cattle. For this reason, 1124 fecal samples were collected from cattle in Markazi provinces of Iran. Selective specific culture media for Salmonella were used to grow a number of isolates from the cattle samples. Salmonella bacteria were identified with biochemical test. The antimicrobial susceptibility test with disc diffusion method was performed on samples of Salmonella by using a molecular based approach,and it was possible to identify Salmonella sp by amplifying specific genes ’’16s rRNA’’ as a step for identification. Our studies showed that the molecular-based approach are more rapid  for  initial detection of Salmonella SP.

 

Key words: Salmonella, cattle, detection, polymerase chain reaction (PCR), 16s rRNA.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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