African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5182

Full Length Research Paper

Molecular characterization of local strains of Bacillus thuringiensis in the North of Algeria

Kebdani M.*
  • Kebdani M.*
  • Laboratory of Ecology and Management of Naturals Ecosystems, Ecology and Environment Department, Abu Bakr Belkaid University, Imama, Tlemcen, Algeria.
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Mahdjoubi M.
  • Mahdjoubi M.
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization, Higher Institute for Biotechnology, University of Manouba Biotechpole of Sidi Thabet Sidi Thabet, Ariana, Tunisia.
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Cherif A.
  • Cherif A.
  • Laboratory of Biotechnology and Bio-Geo Resources Valorization, Higher Institute for Biotechnology, University of Manouba Biotechpole of Sidi Thabet Sidi Thabet, Ariana, Tunisia.
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Gaouar B. N.
  • Gaouar B. N.
  • Laboratory of Ecology and Management of Naturals Ecosystems, Ecology and Environment Department, Abu Bakr Belkaid University, Imama, Tlemcen, Algeria.
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and Rebiahi S. A.
  • and Rebiahi S. A.
  • Laboratory of Applied Microbiology and Environment, Biology Department, Abu Bakr Belkaid University, Imama, Tlemcen, Algeria.
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  •  Received: 01 February 2016
  •  Accepted: 19 September 2016
  •  Published: 07 December 2016

Abstract

The analysis of soil samples taken from orange groves located in four cities of Northern Algeria allowed the isolation and identification of 24 strains belonging to the group Bacillus cereus, based on their physiological, biochemical and molecular characters investigated. They were divided as follow: 12 strains of Bacillus thuringiensis, 1 strain of Bacillus pumilis, 4 strains of Bacillus cereus, 5 strains of Bacillus subtilis and 2 Bacillus mycoides strains. After the molecular characterization performed with the aid of polymerase chain reaction (PCR) analysis, it was confirmed from the parasporal bodies using a scanning electron microscope that the strains of the identified collection belong to the species, B. thuringiensis.

Key words: Bacillus cereus, Bacillus thuringiensis, Bacillus pumilis, Bacillus subtilis, Bacillus mycoides, polymerase chain reaction, scanning EM.