African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Comparison of three DNA extraction methods for polymerase chain reaction (PCR) analysis of bacterial genomic DNA

Omar B. Ahmed*
  • Omar B. Ahmed*
  • Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute for Hajj and Omraa, Umm Al-Qura University, Makkah, Saudi Arabia.
  • Search for this author on:
  • Google Scholar
Atif H. Asghar
  • Atif H. Asghar
  • Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute for Hajj and Omraa, Umm Al-Qura University, Makkah, Saudi Arabia.
  • Search for this author on:
  • Google Scholar
Mogahid M. Elhassan
  • Mogahid M. Elhassan
  • Department of Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia.
  • Search for this author on:
  • Google Scholar


  •  Accepted: 20 January 2014
  •  Published: 05 February 2014

Abstract

Rapid isolation of high-purity microbial genomic DNA is necessary for genome analysis. In this study, the authors compared a one-hour procedure using a microwave with enzymatic and boiling methods of genomic DNA extraction from Gram-negative and Gram-positive bacteria. High DNA concentration and purity were observed for both MRSA and ESBL strains (80.1 and 91.1 µg/ml; OD260/280, 1.82 and 1.70, respectively) when the extraction protocol included microwave pre-heating. DNA quality was further confirmed by PCR detection of mecA and CTX-M. In conclusion, the microwave-based procedure was rapid, efficient, cost-effective, and applicable for both Gram-positive and Gram-negative bacteria.

Key words: DNA, MRSA, ESBL, gene, enzymatic, boiling, microwave.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 8 No. 6
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES