African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5093

Full Length Research Paper

Identification of endophytic fungi from roots of two Dendrobium species and evaluation of their antibacterial property

Roshani Shrestha
  • Roshani Shrestha
  • Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
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Sujit Shah
  • Sujit Shah
  • Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
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Bijaya Pan
  • Bijaya Pan
  • Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
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  •  Received: 25 June 2018
  •  Accepted: 27 July 2018
  •  Published: 07 August 2018

Abstract

Among all orchids, Dendrobium sp. is considered to have high medicinal value. Dendrobium moniliforme and Dendrobium transparens have immense pharmaceutical, commercial potentiality. However, their fungal endophytes remain unexplored. Isolation and identification of thirteen species of endophytic fungi from the root of D. moniliforme as well as five species from the roots of D. transparens were done. The two endophytic fungi namely Aspergillus flavus and Trichoderma harzianum were common for both plant species. Antimicrobial assay was done against selected human pathogen both gram positive and negative Escherichia coli, Pseudomanas aeruginosa, Staphyllococcus aureus and Staphyllococcus epidermidis. The antimicrobial assay showed the significant role of endophytic fungi to inhibit the bacterial growth. Methanolic extract of DeMon VI and DeMon X were prepared to identify the bioactive compounds. Phenolic and 3-Eicosene,(E)-compounds were identified from extract of DeMon VI whereas Pentadecanoic acid, 14 methyle-, methyl ester and Diethyl Phthalate were identified from extract of DeMon X.

 

Key words: Orchids, Dendrobium sp., endophytic fungi, Human pathogen.