African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

The osmoprotective effect of L-carnitine factor on the bacterial growth of Streptococcus agalactiae and Escherichia coli

Maras Meltem1*, Akman Yildiz2, Yula Erkan3 and Gökmen Tülin3
  1Department of Biology Education, Faculty of Education, Zonguldak Karaelmas University, 67300, Kdz. Eregli, Zonguldak, Turkey. 2Science and Art Center, Adana, Turkey. 3Department of Microbiology, Faculty of Medicine, Çukurova University, Adana, Turkey.
Email: [email protected]

  •  Accepted: 23 June 2010
  •  Published: 18 August 2010

Abstract

 

In response to osmotic stress, gram positive bacteria Streptococcus agalactiae and enterobacteria Escherichia coli accumulates compatible solutes from the medium, stress response systems may enable bacteria to adapt cellular response and survival systems to a variety of stress conditions. S. agalactiae and E. coli strainshave a transporter for carnitine that can respond to osmotic stress by regulating its own activity. Brain heart infusion (BHI) broth medium with 1 mM L-carnitine was the medium with the highest growth to be observed. The growth of the E. coli strain was faster than the growth of S. agalactiae strain. S. agalactiae and E. coli are known as bacteria being hard to grow at the laboratory tests for making clinical diagnosis. The medium with L-carnitine factor used in laboratory tests accelerates the bacterial growth.

 

Key words: Bacterial growth, carnitine, osmoprotectant, Streptococcus agalactiae,Escherichia coli.