African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Production of amylase by bacteria isolated from a cassava waste dumpsite in Minna, Niger State, Nigeria

Oyeleke*, S. B. and Oduwole, A. A.
Department of Microbiology, Federal University of Technology, Minna, Niger State, Nigeria.
Email: [email protected]

  •  Accepted: 28 February 2009
  •  Published: 30 April 2009

Abstract

Ten grams (10 g) of soil sample obtained from a cassava waste dumpsite in Kasuwan Gwandabe, Minna, Niger State, was bacteriologically analyzed. Onegram (1.0 g) of the sample inoculated into a liquid soluble starch medium generated reducing sugar with a concentration of 1.65 mg/ml after 72 h. Characterization of the soluble starch amylases revealed an optimum temperature of activity of 70°C. Optimum pH for activity was between 6.5 and 7.5. The most frequently occurring amylolytic bacteria were Bacillus subtilis (37.5%), followed byBacillus subtilis megaterium and Bacillus coagulans (18.75% each). The least occurring isolates were Merus and Bacillus pumilus (6.25% each). The mean zone of amylolytic activity for the isolates ranged between 2.1 mm for B. subtilisand 1.1 mm for B. pumilus.

 

Key words: Aminolytic, enzymes, amylase, optimum, Bacillus sp.

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