African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Induction of defense response in Indian mustard against Alternaria blight through abiotic inducers

Satguru Prasad Yadav*
  • Satguru Prasad Yadav*
  • Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur-208002, U.P. India
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Rajendra Prasad
  • Rajendra Prasad
  • Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur-208002, U.P. India
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Sonit Kumar
  • Sonit Kumar
  • Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur-208002, U.P. India
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Mohammad Shahid
  • Mohammad Shahid
  • Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur-208002, U.P. India
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Sonika Pandey
  • Sonika Pandey
  • Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur-208002, U.P. India
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Mukesh Srivastava
  • Mukesh Srivastava
  • Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur-208002, U.P. India
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Sunil Kumar
  • Sunil Kumar
  • Department of Agricultural Biotechnology, Narendra Dev University of Agriculture and Technology, Faizabad-224229 U.P. India
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  •  Received: 28 October 2014
  •  Accepted: 12 December 2014
  •  Published: 07 January 2015

Abstract

Soaking seed overnight and foliar spray with salicylic acid, indole acetic acid, metalaxyl, dipotassium hydrogen orthophosphate, hydrogen peroxide, calcium chloride and ferric chloride as inducers provided induced resistance in plant against Alternaria brassicae, resulting in a decline in the disease incidence after 10 days of pathogen inoculation. The minimum disease incidence (14%) was recorded from salicylic acid treated plants. Challenge inoculation with abiotic inducers sensitized the seedlings to produce increased level of soluble proteins. The maximum increase of soluble protein content (24.26 and 24.72 mg/g of fresh leaves) was found in salicylic acid treated plant at 5 and 10 days after pathogen inoculation. Similarly, phenol content was (24.26 and 24.72 mg/g of fresh leaves) also found to be maximum in salicylic acid treated plant at 5 and 10 days of inoculation. Protein profiling by SDS-PAGE revealed that foliar spray with salicylic acid induced the synthesis of new proteins; however, such new proteins were not recorded in control.

Key words: Alternaria blight, biochemical changes, chemical inducers, induced resistance, protein profiling, mustard.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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