African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4998

Full Length Research Paper

Studies on optimization of L-glutaminase production under submerged fermentation from marine Bacillus subtilis JK-79

Jambulingam Kiruthika
  • Jambulingam Kiruthika
  • Department of Industrial Biotechnology, Government College of Technology, Thadagam Road, Coimbatore-641013, Tamil Nadu, India.
  • Google Scholar
Saranya Murugesan
  • Saranya Murugesan
  • Department of Industrial Biotechnology, Government College of Technology, Thadagam Road, Coimbatore-641013, Tamil Nadu, India.
  • Google Scholar


  •  Received: 25 March 2019
  •  Accepted: 06 May 2019
  •  Published: 31 January 2020

Abstract

In the current study, a marine strain Bacillus subtilis JK-79 capable of producing L-glutaminase enzyme, was analyzed for maximal L-glutaminase production. The Plackett-Burman design (PBD) was applied to determine the significant variables. Optimum conditions of the significant variables on L-glutaminase production were determined by the Central Composite Design (CCD) of response surface methodology. Maximum L-glutaminase production of 691.27 U/ml under flask condition was obtained at the predicted optimal values of pH 6.9, fructose 2.1% (w/v), yeast extract 1.25% (w/v), and glutamine 2.47% (w/v). Statistical optimization has enhanced L-glutaminase enzyme production upto 3.48 fold when compared with the basal SWG medium. The results of this study revealed that marine B. subtilis JK-79 could be a promising source for L-glutaminase production.

 

Key words: Bacillus subtilis JK-79, L-gltaminase, response surface methodology, submerged fermentation.