Abstract

In the current study, a marine strain Bacillus subtilis JK-79 capable of producing L-glutaminase enzyme, was analyzed for maximal L-glutaminase production. The Plackett-Burman design (PBD) was applied to determine the significant variables. Optimum conditions of the significant variables on L-glutaminase production were determined by the Central Composite Design (CCD) of response surface methodology. Maximum L-glutaminase production of 691.27 U/ml under flask condition was obtained at the predicted optimal values of pH 6.9, fructose 2.1% (w/v), yeast extract 1.25% (w/v), and glutamine 2.47% (w/v). Statistical optimization has enhanced L-glutaminase enzyme production upto 3.48 fold when compared with the basal SWG medium. The results of this study revealed that marine B. subtilis JK-79 could be a promising source for L-glutaminase production.

Key words: Bacillus subtilis JK-79, L-gltaminase, response surface methodology, submerged fermentation.