African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5240

Full Length Research Paper

Efficacy of plasmid curing agent on Streptomyces longsporesflavns

T. R. Sivashankari
  • T. R. Sivashankari
  • Department of Biotechnology, Faculty of School of Bioengineering, SRM University, Katangulathur, Chennai, Tamilnadu, India.
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K. Sudha
  • K. Sudha
  • Department of Biotechnology, St.Peter's College, Kolenchery
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S. Barathi
  • S. Barathi
  • Department of Biotechnology, Faculty of School of Bioengineering, SRM University, Katangulathur, Chennai, Tamilnadu, India.
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V. Karthikeyan
  • V. Karthikeyan
  • Department of Microbiology, Ragavandra Art and Science College, Chidambaram, Tamilnadu, India.
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  •  Received: 18 July 2015
  •  Accepted: 25 February 2016
  •  Published: 14 May 2016

Abstract

Plasmid-specified traits like lactose metabolism and L-asparginase production could be eliminated from Streptomyces longsporesflavns culture during production of fermented product (Microbial succession). In this study we mainly focus on plasmid profiling and plasmid characterization from S. longsporesflavns isolated from marine soil. Then we observe the efficacy of plasmid curing agent on S. longsporesflavns. Plasmid-free strains and cured derivatives harboring only a single plasmid (6.2 Kbp) were also obtained. Treatment of S. longsporesflavns with novobiocin at concentrations of 2.4 µg/ml could produce a large number of chloramphenicol‑ variants at a very high frequency (4.6%). These curing data confirmed the novobiocine act as an effective curing agent for S. longsporesflavns.

Key words: Antibiotic resistance, curing agent, Streptomyces longsporesflavns, plasmid profile, plasmid curing.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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