Abstract

The study was carried out to determine the prevalence of the brucellosis in cattle in Eldein area, Eastern Darfur state, Western Sudan. Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT) and Competitive Enzyme Linked Immuno Sorbent Assay (cELISA) were used for the diagnosis of the disease.  RBPT showed 21 (8.4%) positive results, SAT showed 50(20%) positive sample and cELISA showed 5(2%) positive samples. Twenty four (9.6%) cows had a history of abortion, 20 (8%) had histories of retained placentas and 3 (1.2%) had knee hygromas. The study revealed that sex, age and breed were not associated with the brucella seropositivity (P>0.05). In this study, three types  of serological test were used, namely RBPT, SATand cELISA and there was significant difference between any two given tests (P<0.05). Using agreement between tests (Kappa Statistic) indicated that RBPT and SAT had moderate agreement (Kappa=0.547), RBPT and cELISA had fair agreement (Kappa=0.364) and SAT and cELISA had slight agreement (Kappa=0.158). cELISA is the most sensitive and reliable test in field.

Key words: Brucellosis, cattle, serological tests, prevalence, Sudan.