African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Resistance profile of bacteria isolated from Broncho alveolar lavage fluid (BALF) of patients with severe acute lung disease at the University Hospital Center of Cocody in Abidjan, Côte d’Ivoire

Sopi Anne-Michèle Sandrine N’chott
  • Sopi Anne-Michèle Sandrine N’chott
  • Laboratory of Biology and Health, UFR Biosciences, Felix Houphouet-Boigny University, 01 BP V 34 Abidjan 01, Côte d’Ivoire.
  • Google Scholar
Victoire Gadou
  • Victoire Gadou
  • Department of Biochemistry-Genetics, UPR Microbiology, UFR of Biological Sciences, Peleforo Gon Coulibaly University, BP 1328 Korhogo, Côte d’Ivoire.
  • Google Scholar
Marc Olivier Koffi
  • Marc Olivier Koffi
  • Department of pneumopthisiology of University Hospital Center of Cocody, BP V13 Abidjan 01, Côte d’Ivoire.
  • Google Scholar
Abalé Anatole Toty
  • Abalé Anatole Toty
  • Pasteur Institute of Côte d'Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire.
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Kigninlman Horo
  • Kigninlman Horo
  • Department of pneumopthisiology of University Hospital Center of Cocody, BP V13 Abidjan 01, Côte d’Ivoire.
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Adèle Kacou-N’douba
  • Adèle Kacou-N’douba
  • Department of Bacteriology-Virology, UFR of Medical Sciences, Felix Houphouet-Boigny University, 01 BP V34 Abidjan 01, Côte d’Ivoire.
  • Google Scholar
Allico Joseph Djaman
  • Allico Joseph Djaman
  • Laboratory of Biology and Health, UFR Biosciences, Felix Houphouet-Boigny University, 01 BP V 34 Abidjan 01, Côte d’Ivoire.
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Hortense Faye-Kette
  • Hortense Faye-Kette
  • Pasteur Institute of Côte d'Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire.
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  •  Received: 09 February 2024
  •  Accepted: 07 May 2024
  •  Published: 31 May 2024

Abstract

This study aimed to characterize the microorganisms responsible for severe acute respiratory infections in adult patients hospitalized at the University Hospital Center of Cocody in Abidjan. The biological material consisted of bronchoalveolar lavage fluid (BALF). Isolation and identification of bacterial strains, along with susceptibility testing, were performed using standard microbiological techniques. The bacteria identified included three strains of Klebsiella pneumoniae (75%) and one strain of Streptococcus pneumoniae (25%). Phenotypic analysis of the isolated strains revealed a β-lactam resistance phenotype, specifically the production of extended-spectrum β-lactamases (ESBL) in K. pneumoniae (66.7%), and a resistance phenotype to macrolides, lincosamides, and streptogramin B (MLSb). The resistance rate of K. pneumoniae strains to ceftriaxone, cefepime, cefotaxime, nalidixic acid, norfloxacin, levofloxacin, gentamicin, and tobramycin was 66.7%. The S. pneumoniae strain showed resistance to norfloxacin, ciprofloxacin, nalidixic acid, trimethoprim-sulfamethoxazole, clindamycin, and erythromycin. This study facilitated the isolation of two different bacterial strains responsible for pneumonia in adult patients.

 

Key words: Pneumopathy, BALF, bacteria, resistance, Abidjan.