African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Metallo-β-lactamase producing Enterobacter cloacae: An emerging threat in neonates

Muhammad Usman Qamar*
  • Muhammad Usman Qamar*
  • Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
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Abdul Hannan
  • Abdul Hannan
  • Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
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Muhammad Usman Arshad
  • Muhammad Usman Arshad
  • Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
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Muhammad Arshad
  • Muhammad Arshad
  • Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
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  •  Accepted: 13 January 2014
  •  Published: 05 February 2014

Abstract

Enterobacter cloacae is not a primary human pathogen but has obtained an important role as a nosocomial pathogen. Acquiring this property of metallo-β-lactamases (MBL) producers, the pathogen becomes very virulent for the susceptible neonates and leaves little choice of antibiotics for its management. Blood samples (n=103) were collected in peadriatic blood culture bottles from suspected neonates aseptically. The samples were processed in BACTEC 9120. Antimicrobial susceptibility testing was performed using Kirby-Bauer disc diffusion method according to CLSI 2009. Carbapenems resistant E. cloacae were further analyzed for the detection of carbapenamase as per CLSI guidelines and MBL production by double disc potentiation method. Of 103 blood cultures specimens, 71 were declared positive by BACTEC 9120 and 91 isolates were recovered. Among these isolates, E. cloacae (n=12) were found to be carbapenems resistant and 10 out of these were carbapenamase and MBL producers. All MBL producing pathogens were pan-drug resistant. The spread of MBL among E. cloacae is an emerging threat particularly in hospitalized neonates.

Key words: Enterobacter cloacae, metallo-β-lactamases (MBL), antimicrobial resistance.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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