African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil

Carlos Vergara Torres-Júnior
  • Carlos Vergara Torres-Júnior
  • Departamento de Solos, Instituto de Agronomia. Universidade Federal Rural do Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, CEP 23890-000. Brazil.
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Jakson Leite
  • Jakson Leite
  • Departamento de Solos, Instituto de Agronomia. Universidade Federal Rural do Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, CEP 23890-000. Brazil.
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Carolina Etienne de Rosália e Silva Santos
  • Carolina Etienne de Rosália e Silva Santos
  • Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, PE, CEP 52171-900. Brazil.
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Paulo Ivan Fernandes-Júnior
  • Paulo Ivan Fernandes-Júnior
  • Embrapa Semiárido, BR 428, km 152, Zona Rural, CEP 56302-970 Petrolina, PE. Brazil.
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Jerri Édson Zilli
  • Jerri Édson Zilli
  • Embrapa Agrobiologia, BR 465, Km 07, Seropédica, RJ, CEP 23890-000. Brazil.
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Norma Gouvêa Rumjanek
  • Norma Gouvêa Rumjanek
  • Embrapa Agrobiologia, BR 465, Km 07, Seropédica, RJ, CEP 23890-000. Brazil.
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Gustavo Ribeiro Xavier*
  • Gustavo Ribeiro Xavier*
  • Embrapa Agrobiologia, BR 465, Km 07, Seropédica, RJ, CEP 23890-000. Brazil.
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  •  Accepted: 13 January 2014
  •  Published: 05 February 2014

Abstract

The use of peanut as a trap-plant for obtaining rhizobia may result in a high diversity of efficient isolates. This study aimed to evaluate the diversity and symbiotic efficiency of peanut rhizobia from the Southeastern region of Brazil. The bacteria were isolated from nodules of two genotypes in soils from the States of São Paulo and Rio de Janeiro, Brazil. The bacteria were authenticated and evaluated regarding to their symbiotic capacity. The rhizobial diversity was evaluated through their culture characteristics on yeast mannitol agar (YMA) culture media and by polymerase chain reaction Box-(PCR). Two selected isolates were characterized phenotypically regarding their capacity to solubilize phosphate, to grow on media with different concentrations of NaCI, pH's and incubation temperatures. For these the 16S rRNA gene sequencing and symbiotic efficiency using non-autoclaved soils were conducted. The majority of the obtained isolates showed rapid growth and acidified the culture medium. Analysis of the isolates through the Box-PCR revealed low similarity to the reference strain indicated for the culture and a large genetic variability of the obtained isolates. The isolates AM 01 and AM 07, which clustered with Bradyrhizobium and Rhizobium genus, respectively, showed a potential for evaluations and a means to recommend new rhizobia strains for peanut.

Key words: Arachis hypogaea L., inoculant, biological nitrogen fixation.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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