African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5062

Full Length Research Paper

Resistance of Candida albicans to antifungal drugs in Abidjan (Cote d’Ivoire)

C. G. Kouadio-Yapo
  • C. G. Kouadio-Yapo
  • Laboratoire de Parasitologie-Mycologie, UFR Sciences Médicales, B.P.V 166 Abidjan, Côte d’Ivoire.
  • Google Scholar
N. A. D. Aka
  • N. A. D. Aka
  • Laboratoire de Parasitologie-Mycologie, UFR Sciences Médicales, B.P.V 166 Abidjan, Côte d’Ivoire.
  • Google Scholar
A. V. Bonouman-Ira
  • A. V. Bonouman-Ira
  • Institut Pasteur, Côte d’Ivoire.
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G. S. P. Dou
  • G. S. P. Dou
  • Laboratoire de Parasitologie-Mycologie, UFR Sciences Médicales, B.P.V 166 Abidjan, Côte d’Ivoire.
  • Google Scholar
K. H. G. Sonan
  • K. H. G. Sonan
  • Département de Génétique, Université PGC, B.P. 1328 Korhogo, Côte d’Ivoire.
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K. D. Zika
  • K. D. Zika
  • Laboratoire de Parasitologie-Mycologie, UFR Sciences médicales, B.P.V 18 Bouaké, Côte d’Ivoire.
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K. D. Adoubryn
  • K. D. Adoubryn
  • Laboratoire de Parasitologie-Mycologie, UFR Sciences médicales, B.P.V 18 Bouaké, Côte d’Ivoire.
  • Google Scholar
M. Dosso
  • M. Dosso
  • Institut Pasteur, Côte d’Ivoire.
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  •  Received: 11 February 2018
  •  Accepted: 17 February 2020
  •  Published: 31 October 2020

Abstract

This study aimed to evaluate the resistance levels of strains of Candida albicans to the antifungals commonly used in Abidjan, Cote d’Ivoire. This is a prospective study that was carried out from July to October 2017 at the mycology laboratory of the Institut Pasteur of Cote d’Ivoire. 105 Candida isolates, obtained from swabs taken from patients receiving out-patient treatment, were seeded on chromogenic medium. Identification of Candida species was carried out by MALDI-TOF mass spectrometry (Vitek MS bioMérieux). The susceptibility of C. albicans strains to 5-fluorocytosine, amphotericin B, fluconazole, itraconazole and voriconazole was evaluated using the microdilution technique in a semi-solid medium to determine the minimum inhibitory concentration with the ATB1 Fungus 3 kit. Out of 105 Candida strains, 68 (64.8%), including C. albicans, were identified on the chromogenic medium and confirmed by MALDI-TOF spectrometry. These C. albicans strains exhibited varying levels of resistance to the antifungals tested: 1.5% for 5-fluorocytosine, 26.3% for fluconazole, 39.7% for itraconazole, 27.9% for voriconazole. No resistance to amphotericin B was observed. C. albicans strains taken from ear pus swabs exhibited greater resistance (P = 0.0113). C. albicans is developing increasing resistance to common antifungals, hence the need for regular surveillance in resource-poor countries.

 

Key words: Candidiasis, Candida albicans, resistance, mycosis, antifungual drug.