African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5123

Full Length Research Paper

Molecular characterization of water-borne multi-drug resistant Escherichia coli

Stephen T. Odonkor
  • Stephen T. Odonkor
  • School of Public Services and Governance, Ghana Institute of Management and Public Administration Accra, Ghana.
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Kennedy K. Addo
  • Kennedy K. Addo
  • Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
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Anthony M. Sallar
  • Anthony M. Sallar
  • School of Liberal Arts & Social Sciences, Ghana Institute of Management and Public Administration Accra, Ghana.
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  •  Received: 16 February 2018
  •  Accepted: 02 January 2019
  •  Published: 30 April 2021

Abstract

Antibiotics are very important in the fight against infectious disease caused by bacteria and other microbes for decades. Today microbes have developed ways to resist antimicrobial agents targeted at them. We sought to characterize and simultaneously detect virulence genes associated water-borne antibiotic resistant Escherichia coli, obtained from water sampled from ground and surface water sources. The Analytical Profile Index (API) was use for the identifications of E. coli isolates. The Kirby-Bauer disk diffusion method was employed for susceptibility testing. A DNA•STRIP molecular assay technology designed for detection of shiga toxin genes was used for the molecular characterization. E. coli isolates showed a high (32.99%), resistance to penicillin, and was highly susceptible (93.8%) to nitrofurantoin. E. coli was confirmed Polymerase Chain Reaction (PCR). None of the confirmed multidrug resistant E. coli isolate had genes for stx1 and stx2. However, the eae intimin virulence gene was found on 6% of the multidrug resistant E. coli isolates. It was concluded that E. coli has developed a very high resistance to the various antibiotics. Second, the genotype EHEC test based on the DNA•STRIP technology used in this study has proved to be efficient and reliable in the molecular characterization of the multi-drug resistant E. coli isolates. The DNA•STRIP Genotype EHEC technology test is therefore recommended for pathogenic E. coli detection and monitoring. There is also a need to revise strategies towards the multidrug resistance programme.

 

Key words: DNA•STRIP Technology, Escherichia coli, polymerase chain reaction, drug resistance, water bourne.