Abstract

Antimicrobial activities were evaluated from the known varied weights of rose petals (50 to 400 mg) and pollens extracts of Rosa damascena cv. Taifi in different aqueous solutions and solvents such as sterilized zamzam water, distilled water, ethanol, acetone, isoamyl alcohol and 100 millimolar (mM) Tris-HCl at pH 8.7 by using liquid nitrogen. The highest activity was recorded against ethyl alcohol and acetone extracts of R. damascena cv. Taifi. The roses were obtained from Al-Kamal factory from Al-Hada and Mohammed Bin Othman Farm in Abha. The research was conducted at the Department of Biotechnology, Taif University from February to May 2013. The bacterial strains from ATCC and the nutrient agar were utilized for the study. Experiment results shows that the ethyl alcohol and acetone extract of rose's petals and pollen have shown an activity against Pseudomonas aeruginosa (ATCC 27853). The acetone extract of rose's petals showed activity against Candida albicans (ATCC 14053) and Pseudomanas aeruginosa (ATCC 27853). Also, the ethyl alcohol extract of rose's petals had shown activity against Escherichia coli (ATCC 25922). The water extract of rose petals showed an activity against C. albicans (ATCC 14053) 200 mg/ml. The acetone extract of rose's petals appeared to have a moderate activity against C. albicans (ATCC 14053) and showed the highest antimicrobial activities against P. aeruginosa (ATCC 27853). Encouraging results were due to the fact that the plants are rich antibacterial sources and further study should be carried out to confirm the purification of the antibacterial compounds.

Key words: Agar well diffusion method, antimicrobial activities, medicinal plant extract.