Abstract

Klebsiella pneumoniae and Enterobacter sp. are emerging as major health problems world-wide. UTI is a significant problem in children, as well as adults and requires a large scale study at regular intervals in order to identify organisms from time to time and recommend prompt treatment to reduce UTI related morbidity and mortality in humans. In the present study, organisms were isolated from urine samples of patients suspected for UTI. The isolated organisms were identified based on biochemical and molecular analyses and were characterized. They were identified as K. pneumoniae SSN, K. pneumoniae KSS and Enterobacter sp. SCSS. Also genetic variations between the twoKlebsiella sp. were studied by virtual digestion using different restriction enzymes.

Key words: UTI, Klebsiella pneumoniae, Enterobacter sp., 16S rRNA, biochemical characterization.