African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5188

Full Length Research Paper

Molecular characterization and virulence of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella (Lepidoptera: Pyralidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) larvae

Margy A. Esparza Mora
  • Margy A. Esparza Mora
  • Plant Health and Applied Biotechnology Program - Mycology Laboratory - Veterinary Institute - Federal Rural University of Rio de Janeiro - BR 465 Km 07 - ZIP. 23890-000, Seropédica – RJ, Brazil.
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Julie G. Chacón-Orozco
  • Julie G. Chacón-Orozco
  • Health, Food Safety and Environmental in Agribusiness - Instituto Biológico, São Paulo, SP, Brazil.
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Ricardo Harakava
  • Ricardo Harakava
  • Phytopathology and Biochemistry Laboratory, Instituto Biológico, São Paulo, SP, Brazil.
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Janaina Ribeiro Costa Rouws
  • Janaina Ribeiro Costa Rouws
  • Genetics and Breeding Plants - EMBRAPA Agrobiologia – BR 465, Km 07 - ZIP. 23891-000, Seropédica – RJ, Brazil.
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Marcelo E. Fraga
  • Marcelo E. Fraga
  • Veterinary Science - Mycological Research Center and Micotoxicológica - Veterinary Institute - Federal Rural University of Rio Janeiro - BR 265 Km 07 - ZIP. 23890-000, Seropédica - RJ, Brazil.
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  •  Received: 24 February 2016
  •  Accepted: 08 April 2016
  •  Published: 21 May 2016

Abstract

Entomopathogenic fungi considerably vary in their action and virulence mode. The contamination mainly depends on the adhesion and penetration of the fungus in the host integument. Four isolates from Beauveria bassiana and Metarhizium anisopliae were molecularly characterized by rDNA-ITS sequencing. Their virulence against last instar larvae of Galleria mellonella and Tenebrio molitor at four conidia concentrations: 1×106, 1×107, 1×108 and 1×109 conidia.ml-1 were in vitro evaluated and the lethal concentrations (LC50 and LC90) as lethal time (LT50 and LT90) were determined. Sequencing of rDNA-ITS 500 bp fragments allowed the identification of Ma10MI, Ma12MI, Ma58MI and Ma11MI isolates as M. anisopliae and Bb11MI, Bb79MI, Bb53MI and Bb27MI isolates as B. bassiana by sequence comparison to GenBank. According to the pathogenicity test, B. bassiana strains with higher performance for G. mellonella were Bb53MI and Bb79MI with LC50= 3.98×107 and 1.04×107 conidia.ml-1 and LT50= 5.46 and 5.27 days, respectively, as well as to T. molitor was Bb79MI with LC50= 1.03×107 conidia.ml-1 and LT50= 5.57 days. The M. anisopliae strains with the best performance were Ma58MI and Ma10MI both T. molitor (LC50= 1.0×106 and 1.0×107 conidia.ml-1 and LT50= 4.06 and 5.22 days, respectively) and G. mellonella (LC50= 6.3×107 and 1.1×108 conidia.ml-1 and LT50= 5.18 and 6.37 days, respectively), these isolates might be considered as new promising candidates for the microbial pest control.

 

Key words: Pathogenicity, molecular taxonomy, entomopathogenic fungi, microbial control.