African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Studies on exopolysaccharide and indole acetic acid production by Rhizobium strains from Indigofera

B. Saritha Kumari, M. Raghu Ram* and K. V. Mallaiah
Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar-522 510, Guntur, A. P., India.
Email: [email protected]

  •  Accepted: 09 October 2008
  •  Published: 31 January 2009

Abstract

Rhizobium strains were isolated from root nodules of five species of Indigoferaviz., Indigofera trita, Indigofera linnaei, Indigofera astragalina, Indigofera parviflora and Indigofera viscosa on Yeast Extract Mannitol Agar (YEMA) medium. The strains were examined for production of acid, exopolysaccharide (EPS) and indole acetic acid (IAA) by utilizing 10 different carbon sources. The Rhizobium strains isolated from I. trita, I. parviflora and I. viscosa showed maximum growth on glucose, while those from I. linnaei and I. astragalina showed maximum growth on fructose and maltose, respectively. All the five strains produced acid, EPS and IAA in Yeast extract mannitol broth. Among the strains studied, maximum EPS production was observed with the strain isolated from I. parviflora and maximum IAA production was observed with the strain isolated from I. viscosa.

 

Key words: Rhizobium, Indigofera species, exopolysaccharide, indole acetic acid, acid production.

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