Full Length Research Paper
ABSTRACT
Head rot of cabbage caused by Sclerotinia sclerotiorum leads to rotting of fully matured cabbage heads in the field. In the present study the antagonistic effects of twenty Bacillus isolates was tested against S. sclerotiorum in vitro. Eight effective Bacillus isolates obtained from studies in vitro, commercial formulations of Trichoderma viride isolate TV-1 and Pseudomonas fluoroscens isolate Pf-1along with a fungicide check (Nativo-Tebuconazole+Trifloxystrobin) were carried further for field studies. Results of field studies indicated that fungicide check of Nativo (1.5 g/L) was highly effective with least disease incidence of 10.36% indicating 74.50% reduction over control. Among the biocontrol agents commercial formulation of Trichoderma viride isolate TV-1 was the most effective showing disease incidence of 11.38% indicating 72.00% reduction over control followed by Bacillus amyloliquefaciens isolate B15 and Pseudomonas fluoroscens isolate Pf-1 showing disease incidence of 13.24 and 13.31% indicating 67.41 and 67.24% reduction over control respectively and both treatments were on par. B. licheniformis isolate B16 was found to be least effective with 20.41 percent disease incidence indicating 49.76% reduction over control.
Key words: Bacillus, commercial formulation, fungicide check, Pseudomonas fluoroscens, Trichoderma viride, Sclerotinia sclerotiorum.
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONFLICT OF INTERESTS
REFERENCES
Abdullah MT, Ali NY, Patrice S (2008). Biological control of Sclerotinia sclerotiorum (Lib.) de Bary with Trichoderma harzianum and Bacillus amyloliquefaciens. Crop Prot. 27:1354-1359. |
|
Alvarez F, Castro M , Príncipe A, Borioli G, Fischer S, Mori G, Jofré E (2012).The plant-associated Bacillus amyloliquefaciens strains MEP218 and ARP23 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of Sclerotinia stem rot disease. J. Appl. Microbiol. 112:159-174. |
|
Boyetchko S, Pedersen E, Punja Z, Reddy M (1999). Formulations of biopesticides. Methods Biotechnol. 5:487-508. |
|
Collins DP, Jacobsen BJ (2003). Optimizing a Bacillus subtilis isolate for biological control of sugarbeet cercospora leaf spot. Biol. Control 26:153-161. |
|
Farr DF, Bills GF, Chamuris GP, Rossman AY (1989). Fungi on Plants and Plant Products in the United States. In. The American Phytopathological Society. P 1252. |
|
Fernando PM, Larissa CF, Leandro PE, Paulo, ES (2013). Antagonism of Bacillus subtilis against Sclerotinia sclerotiorum on Lactuca sativa. J. Agric. Sci. 5(4):214-223. |
|
Fernando WGD, Nakkeeran S, Zhang Y, Savchuk S (2007). Biological control of Sclerotinia sclerotiorum (Lib.) de Bary by Pseudomonas and Bacillus species on canola petals. Crop Prot. 26(2):100-107. |
|
Gaur RB, Sharma RN, Gautam VS, Dangi RP (2010). Management of Sclerotinia rot of mustard through bioagents. Indian Phytopathol. 63:392-397. |
|
Gomez KA, Gomez AA (1984). Statistical Procedure for Agricultural Research. John Wiley and Sons, New York. |
|
Grau CR (1988). Soybean diseases of the north central region. In. American Phytopathological Society. pp. 56-66. |
|
Hudyncia J, Shew HD, Cody BR, Cubeta MA (2000). Evaluation of wounds as a factor to infection of cabbage by ascospores of Sclerotinia sclerotiorum. Plant Dis. 84(3):316-320. |
|
Li H, Li H, Bai Y, Wang J, Nie M, Li B, Xiao M (2011). The use of Pseudomonas fluoroscens P13 to control Sclerotinia stem rot (Sclerotinia sclerotiorum) of oilseed rape. J. Microbiol. 49(6):884-889. |
|
Pankaj K, Dubey RC, Maheshwari DK (2012). Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens. Microbiol. Res. 167:493-499. |
|
Purdy LH (1979). Sclerotinia sclerotiorum:History, diseases and symptomatology, host range, geographic distribution and impact. Phytopathology 69:875-880. |
|
Sharie R, Mojdeh M, Dariush S (2013). The use of Bacillus amyloliquefaciens to control Sclerotinia stem rot (Sclerotinia sclerotiorum) of cucumber. Int. J. Farm. Allied Sci. 2:965-970. |
|
Shoda M (2000). Bacterial control of plant diseases. J. Biosci. Bioeng. 89:515-521. |
|
Vipin MD, Sandhya M, Vasvi C, Swati T, Poonam S, Sumit Y, Shashank M, Ijinu TP, Varghese G, Ajit V, Chandrashekhar N (2012). Biocontrol and plant growth promotion and conferring stress tolerance: Multifaceted role of Bacillus licheniformis 9555. J. Fitopatolgi 3(4):780-783. |
|
Xiaojia H, Daniel PR, Lihua X, Jude EM, Changbing Y, Yinshiu L, Shujie Z, Xing L (2013). Bacillus megaterium A6 supresses Sclerotinia sclerotiorum on oilseed rape in the field and promotes oilseed rape growth. Crop Prot. 52:151-158. |
Copyright © 2024 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0