African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Article in Press

Diagnostic value of 2 HIV rapid diagnostic tests 16 years after their adoption in national strategies in the Central African Republic (CAR)

Christian Diamant Mossoro-Kpinde, Thierry Mbesse, Christielle Bobossi, Coretha Bokia-Baguida, Simplice Arthur Sombot, Nina Esther Ontsira Ngoyi, Hermione Dahlia Mossoro-Kpinde, Rosine Feissona, Gérard Gresenguet

  •  Received: 23 June 2021
  •  Accepted: 15 September 2021
Objective: The broad genetic diversity of HIV requires regular reassessment of adopted HIV diagnostic tests. The objective of this study was to reassess the performances of Determine® HIV 1/2 and Uni-gold® HIV1/2, 2 HIV rapid diagnostic tests of the national algorithm, since 2005 in Central African Republic. Methods: A reference prospective panel of 150 plasma samples were tested in duplicate with Determine® HIV 1/2 and Uni-gold® HIV, according to reference serological immune-enzymatic method. Analytical and Virological characteristics were calculated. Results: Sensitivity, specificity, positive and negative predictive value (PPV, NPV) of Determine® HIV 1/2 were 100%, as well as the specificity and NPV of Uni-gold® HIV 1/2. Uni-gold® HIV 1/2 sensitivity and PPV were 96% and 92%, respectively. The Cohen k coefficient was close to 1 for the 2 tests, 1 for Determine® HIV 1/2 and 0.97 for Uni-gold® HIV 1/2. Conclusion: Except for the sensitivity of Uni-gold® HIV 1/2, the performances of 2 tests were acceptable and in perfect agreement with the reference tests. These results require a revision of the algorithm. In addition, an exploration in molecular biology is indicated to determine the subtypes of the HIV strains of the 4 samples that tested false negative with Uni-gold® HIV 1/2.

Keywords: HIV, rapid diagnosis test, sensitivity, specificity, Africa, Central African Republic.