African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5056

Article in Press

Purification of a Saponin with antifungal activity isolated from the seeds of Filicium decipiens plant: Hemolytic inhibition activity by the Lectin PpyLL

BRANDO-COSTA ROMERO, Ferreira Araújo Vivianne

  •  Received: 25 August 2015
  •  Accepted: 12 February 2016
Triterpenoids are a large group of naturally occurring substances in plant kingdon. In this paper was presented a Saponin from Fillicium decipiens seeds purified through a protocol using Ion-exchange coupled-chromatography for protein retention followed by Gel filtration under Sephadex-LH-20, and characterized by HPLC-MS. Saponin was examined for its antifungal properties by sensibility and dilution methods, and submitted to Hemolytic experiment using different red cells (horse, cow, rabbit, chicken, rat, and Human ABO system). Sephadex-LH-20 and confirmed by TLC showed a purity degree of three saponins with high resolution, also one of them presenting a peak at 752.35g/mol. This major-peak of Saponin showed selective toxicity against Aspergillus flavus, presenting low levels of minimum fungicidal concentration, minimum inhibitory concentration and IC50 at 12.8g/mL, 7.5g/mL and 5.8g/mL, respectively. Under hemolytic induction, it was observed that red cells from rabbit and cow were more affect by Saponin. Added to this study, we examined the hemolytic effect of this Saponin in the presence of PpyLL, a Lectin, and it was observed a significantly decrease on hemolysis. These characteristics provide us a fundamental knowledge in the F. decipiens secondary metabolites studies which would benefit future research of Biocontrol in biopesticides field against Aspergillus flavus.

Keywords: Purification; saponin; triterpenes; antifungal activity; Hemolysis.