This study was undertaken to explore the antimicrobial resistance of the organism Pseudomonas to the diverse classes of antimicrobials formulated in clinical practice and the prevalence of metallo-Beta-lactamases (mbls) produced by these isolates. Aims and objectives was to analyze the antibiotic resistance .The 97 strains were isolated both clinically and environmentally and were characterized by 16S molecular characterization. Segregating the clinical and environmental isolates, the antimicrobial resistance for the environmental isolates was found as Imepenem-1.08% rest. Strains and 9.36% senstv. Strains; Gentamicin- 2.88% sentv. Strains and 9.36% rest. strains; Netilmicin â€“ 1.44% Senstv. strains and rest. 9.72% and antibiotic profiles for the clinical isolates was-Imepenem-3% rest., strains and 28.8% senstv. strains; Gentamicin-6.6% sentv. strains and 24.6% rest. strains ; Netilmicin â€“ 7.2% senstv. strains and 26.4% rest. strains. Despite of the risk of Pseudomonas manifestations there has been no data available on its prevalence, epidemiology, and antibiotic resistance profile in Barak Valley region. Therefore the present study envisages the prevalence of the Pseudomonas in this region and its extensive resistance to the range of antibiotics administered today by the clinical practitioners.