African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Helicobacter pylori vacA genotyping in relation to cagA status, ultra-structure of gastric mucosa and clinical outcomes in Egyptian patients

Manal Diab
  • Manal Diab
  • Microbiology Department, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt.
  • Google Scholar
Mohamed Shemis
  • Mohamed Shemis
  • Biochemistry Department, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt.
  • Google Scholar
Maged El-Ghannam
  • Maged El-Ghannam
  • Gastroentrology and Hepatology Department, Theodor Bilharz Research Institute (TBRI). Guiza, Egypt.
  • Google Scholar
Doaa Gamal
  • Doaa Gamal
  • Microbiology Department, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt.
  • Google Scholar
Magda Azmy
  • Magda Azmy
  • Electron Microscopy Department, Theodor Bilharz Research Institute (TBRI) Guiza, Egypt.
  • Google Scholar
Dalia Salem
  • Dalia Salem
  • Microbiology Department, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt.
  • Google Scholar
Soheir Mansy
  • Soheir Mansy
  • Electron Microscopy Department, Theodor Bilharz Research Institute (TBRI) Guiza, Egypt.
  • Google Scholar
Mohamed Saber
  • Mohamed Saber
  • Biochemistry Department, Theodor Bilharz Research Institute (TBRI), Guiza, Egypt.
  • Google Scholar


  •  Received: 02 August 2015
  •  Accepted: 12 February 2016
  •  Published: 14 April 2016

References

Abu Amra M (2010). Determination of cagA and vacA genotypes and evaluation of clarithromycin resistance of Helicobacter pylori infections in Gaza Strip, Palestine. Master thesis of Biological Science Medical Technology.

 

Al Quabandi A, Mustafa A, Siddique I, Khajah AK, Madda JP, Junaid TA (2005). Distribution of vacA and cagA genotypes of Helicobacter pylori in Kuwait. Acta. Trop. 93(3):283-288.
Crossref

 
 

Amer F, El-Sokkary R, Elahmady M, Gheith T, Abdelbary EH, Elnagar Y, Abdalla WM (2013). Helicobacter pylori genotypes among patients in a university hospital in Egypt: Identifying the determinants of disease severity. J. Microbiol. Inf. Dis. 3(3):109-115.
Crossref

 
 

Argent RH, Thomas RJ, Letley DP, Rittig MG, Hardie KR, Atherton JC (2008). Functional association between the Helicobacter pylori virulence factors VacA and CagA. J. Med. Microbiol. 57:145-150. http://dx.doi: 10.1099/jmm.0.47465-0 PMid:18201978
Crossref

 
 

Atherton JC, Cao P, Peek RM Jr, Tummuru MK, Blaser MJ, Cover TL (1995). Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA types with cytotoxin production and peptic ulceration. J. Biol. Chem. 28(30):17771-17777.

 
 

Bai H, Li Q, Liu X, Li Y (2010). Characteristics and interactions of Helicobacter pylori and H. pylori-infected human gastroduodenal epithelium in peptic ulcer: A transmission electron microscopy study. Dig. Dis. Sci. 55(1):82-88.
Crossref

 
 

Benenson S1, Halle D, Rudensky B, Faber J, Schlesinger Y, Branski D, Rabinowitz N, Wilschanski M (2002). Helicobacter pylori genotypes in Israeli children: The significance of geography. J. Pediatr. Gastroenterol. Nutr. 35:680-684.
Crossref

 
 

Chisholm SA, Owen RJ, Teare EL, Saverymuttu S (2001). PCR-based diagnosis of Helicobacter pylori infection and real-time determination of clarithromycin resistance directly from human gastric biopsy samples. J. Clin. Microbiol. 39:1217-1220
Crossref

 
 

Chung C, Olivares A, Torres E, Yilmaz O, Cohen H, Perez-Perez G (2010). Diversity of vacA intermediate region among Helicobacter pylori strains from several regions of the world. J. Clin. Microbiol. 48:690-696
Crossref

 
 

Diab M, Saad El-Dine S, Aboul-Fadl L, Shemis M, Omran Z, Badawi A, El-Ghannam M, El-Ray A, Fam N, El-Defrawy I, El-Sherbini E, Saber M (2009). Helicobacter pylori cag pathogenicity island genes among dyspeptic patients with chronic gastritis. Egypt. J. Med. Microbiol. 18(9):43-53.

 
 

El-Gharbawy M, Helmy M, Imam I (2006). Association of CagA and VacA genotypes of Helicobacter pylori with gastroduodenal diseases. Egypt. J. Med. Microbiol. 15:3589-597.

 
 

Falsafi T, Favaedi R, Mahjoub F, Najafi M (2009). Application of stool-PCR test for diagnosis of Helicobacter pylori infection in children. World J. Gastroenterol. 15(4):484-488.
Crossref

 
 

Ferreira R, Machado J, Figueiredo C (2014). Clinical relevance of Helicobacter pylori vacA and cagA genotypes in gastric carcinoma. Best Pract. Res. Clin. Gastroentrol. 28:1003-1015.
Crossref

 
 

Hussein NR (2010). Helicobacter pylori and gastric cancer in the Middle East: A new enigma? World J. Gastroenterol. 16:3226-3234.
Crossref

 
 

Khalifa M, Sharaf R, Aziz R (2010). Helicobacter pylori: A poor man's gut pathogen? Gut Pathog. 2:2.
Crossref

 
 

Kim SY, Woo CW, Lee YM, Lee YM, Son BR, Kim JW, Chae HB, Youn SJ, Park SM. (2001). Genotyping cagA, vacA subtype, iceA1, and babA of Helicobacter pylori isolates from Korean patients, and their association with gastroduodenal diseases. J. Kor. Med. Sci. 16:579-584.
Crossref

 
 

Krashias G, Bashiardes S, Potamitou A, Potamitis GS, Christodoulou C (2013). Prevalence of Helicobacter pylori cagA and vacA genes in Cypriot patients. J. Infect. Dev. Ctries. 7(9):642-650.
Crossref

 
 

Letley DP, Rhead, JL, Twells RJ, Dove B, Atherton JC (2003). Determinants of non-toxicity in the gastric pathogen Helicobacter pylori. J. Biol. Chem. 278:26734-26741.
Crossref

 
 

Marie M. (2012) Relationship between Helicobacter pylori virulence genes and clinical outcomes in Saudi Patients. J. Kor. Med. Sci. 27:190-199.
Crossref

 
 

Nimri L, Matalka I, Bani Hani K, Ibrahim M (2006). Helicobacter pylori genotypes identified in gastric biopsy specimens from Jordanian patients. BMC Gastroenterol. 6:27.
Crossref

 
 

Ozbey G, Aygun C (2012). Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome. Braz. J. Microbiol. 43(4):1332-1339.
Crossref

 
 

Palframan L, Kwok T, Gabriel K (2012). Vacuolating cytotoxin A (VacA), a key toxin for Helicobacter pylori pathogenesis. Cellul. Inf. Microbiol. 2:92.
Crossref

 
 

Ramelah M, Aminuddin A, Alfizah H (2005). cagA gene variants in Malaysian Helicobacter pylori strains isolated from patients of different ethnic groups. FEMS Immunol. Med. Microbiol. 44:239-242.
Crossref

 
 

Sugimoto M, Yamaoka Y (2009).The association of vacA genotype and Helicobacter pylori-related disease in Latin American and African populations. Clin. Microbiol. Infect. 15(9):835-842.
Crossref

 
 

Van Doorn LJ, Figueiredo C, Mégraud F, Pena S, Midolo P, Queiroz DM, Carneiro F, Vanderborght B, Pegado MD, Sanna R, De Boer W, Schneeberger PM, Correa P, Ng EK, Atherton J, Blaser MJ, Quint WG (1999). Geographic distribution of vacA allelic types of Helicobacter pylori. Gastroenterology 116:823-830.
Crossref

 
 

Warren J, Marshall B (1983). Unidentified curved bacilli on gastric epithelium in active gastritis. Lancet 1:1273-1275.

 
 

Zhou J, Zhang J, Xu C, He L (2004). cagA genotype and variants in Chinese Helicobacter pylori strains and relationship to gastroduodenal diseases. J. Med. Microbiol. 53(3):231-235.
Crossref