African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5235

Full Length Research Paper

Prevalence, risk factors and multidrug resistance profile of Staphylococcus aureus isolated from bovine mastitis in selected dairy farms in and around Asella town, Arsi Zone, South Eastern Ethiopia

Kemal Kedir Elemo
  • Kemal Kedir Elemo
  • College of Agriculture and natural resources, Animal and Range Sciences Course Team, Madda Walabu University, Bale-Robe, Ethiopia.
  • Google Scholar
Tesfaye Sisay
  • Tesfaye Sisay
  • College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
  • Google Scholar
Ashanafi Shiferaw
  • Ashanafi Shiferaw
  • College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
  • Google Scholar
Muhammadhussien Aman Fato
  • Muhammadhussien Aman Fato
  • Bale Zone Live Stock and Fishery Development Office, Bale-Robe, Ethiopia.
  • Google Scholar


  •  Received: 17 March 2017
  •  Accepted: 17 July 2017
  •  Published: 07 December 2017

References

Abebe M, Daniel A, Yimtubezinash W, Genene T (2013). Identification and antimicrobial susceptibility of Staphylococcus aureus isolated from milk samples of dairy cows and nasal swabs of farm workers in selected dairy farms around Addis Ababa, Ethiopia. Afr. J. Microbiol. Res. 7(27):3501-3510.

 

Abera M, Elias B, Aragaw K, Denberga Y, Amenu K, and Sheferaw D (2012). Major causes of mastitis and associated risk factors in smallholder dairy cows in Shashemene, southern Ethiopia. Afr. J. Agric. Res. 7(24): 3513-3518.

 
 

Abera M, Demie B, Aragaw K, Regassa F, Regassa A (2010). Isolation and identification of Staphylococcus aureus from bovine mastitic milk and their drug resistance patterns in Adama town, Ethiopia. J. Vet. Med. Anim. Health 2(3): 29-34.

 
 

Abo-shama UH (2014). Prevalence and antimicrobial susceptibility of Staphylococcus aureus isolated from cattle, buffalo, sheep and goat`s raws milk in Sohag governorate, Egypt. Assiut Vet. Med. J. 60:141.

 
 

Alekish M O, Al-Qudah KM, Al-Saleh A (2013). Prevalence of antimicrobial resistance among bacterial pathogens isolated from bovine mastitis in Northern Jordan. Revue Méd. Vét. 164(6):319-326.

 
 

Alemu G (2010). Incidence of selected contagious bovine mastitis pathogens and drug resistance patterns of S. aureus in Hawassa. DVM thesis, University of Gondar, Faculty of Veterinary Medicine.

 
 

Anderson AD, Nelson JM, Rossiter S, Angulo FJ (2003). Public health consequences of use of antimicrobial agents in food animals in the United States. Microb Drug Resist. 9:373-379.
Crossref

 
 

Andrew AH, Blowey RW, Eddy RG (2004). Bovine disease, medicine and husbandry of cattle.2nd ed. Blackwell Science Ltd. pp. 326-360.

 
 

Angulo FJ, Nargund VN, Chiller TC (2004). Evidence of an association between use of anti-microbial agents in food animals and anti-microbial resistance among bacteria isolated from humans and the human health consequences of such resistance. J Vet Med B Infect. Dis Vet. Public Health 51: 374-379.
Crossref

 
 

Ateba, CN.,Mbewe, M., Moneoang, MS. and Bezuidenhout, CC. (2010): Antibiotic-resistant Staphylococcus aureus isolated from milk in the Mafikeng Area, North West province, South Africa. S Afr. J. Sci. 106(11/12): 243.
Crossref

 
 

Batabyal B, Kundu GKR, Biswas S (2012). Methicillin-resistant Staphylococcus aureus: A brief review. Int. Res. J. Biol. Sci. 1:65-71.

 
 

Biffa D, Debela E, Beyene F (2005). Prevalence and risk factors of mastitis in lactating dairy cows in Southern Ethiopia. Int. J. Appl. Res. Vet. Med. 3(3):189-198.

 
 

Broekema NM, Van TT, Monson TA, Marshall SA, Warshauer DM (2009). Comparison of cefoxitin and oxacillin disk diffusion methods for detection of mecA mediated resistance in Staphylococcus aureus in a large-scale study. J. Clin. Microbiol. 47:217-219.
Crossref

 
 

Carbon C (2000). MRSA and MRSE: Is there an answer? Clin. Microbiol. Infect. 6: 17-22.
Crossref

 
 

Carter G, Chengappa M (1991). Essentials of Veterinary Bacteriology and Mycology 4thEd, Lea and Febiger, USA. 290 p.

 
 

CLSI (2008). Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals: approved standard. 3rded. CLSI, Wayne.

 
 

Cookson BD, Phillips I (1998). Epidemic MRSA. J. Antimicrob. Chemother. 21:57-65.
Crossref

 
 

Cosgrove SE, Sakoulas G, Perencevich EN, Schwaber MJ, Karchmer AW, Carmeli Y (2003). Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin. Infect. Dis. 36:53-59.
Crossref

 
 

Cuny C, Wieler LH, Witte W (2015). Livestock-associated MRSA: The impact on humans. Antibiotics 4: 521-543.
Crossref

 
 

Daka D, G/Silassie S, Yihdego D (2012). Antibiotic-resistance Staphylococcus aureus isolated from cow's milk in the Hawassa area, South Ethiopia. Ann. Clin. Microbiol. Antimicrob. 11:26.
Crossref

 
 

de Medeiros ES, França CA, Krewer Cda C, Peixoto Rde M, de Souza AF Jr, Cavalcante MB, da Costa MM, Mota RA (2011): Antimicrobial resistance of Staphylococcus spp. isolates from cases of mastitis in buffalo in Brazil. J. Vet. Diagn. Invest. 23(4):793-796.
Crossref

 
 

De Neeling AJ, Van den Broek MJM, Spalburg EC, Van Santen-Verheuvel MG, Dam-Deisz W, Boshuizen HC (2007). High prevalence of methicillin resistant Staphylococcus aureus in pigs. Vet. Microbiol. 122:366-372.
Crossref

 
 

Deurenberg RH, Stobberingh EE (2008). The evolution of Staphylococcus aureus. Infect. Genet. Evol. 8:747-763.
Crossref

 
 

Duquette RA, Nuttall TJ (2004). Methicillin-resistant Staphylococcus aureus in dogs and cats: an emerging problem? J. Small Anim. Pract. 45:591-597.
Crossref

 
 

Erskine RJ, Walker RD, Bolin CA, Bartlett PC, White DG (2002). Trends in antibacterial susceptibility of mastitis pathogens during a seven-year period. J. Dairy Sci. 85:1111-1118.
Crossref

 
 

Farzana K, Shah SNH, Jabeen F (2004). Antibiotic resistance pattern against various isolates of Staphylococcus aureus from raw milk samples. J. Res. Sci. 15(2):145-151

 
 

Finch R, Hunter PA (2006). Antibiotic resistance-action to promote new technologies: report of an EU Intergovernmental Conference held in Birmingham, UK. J. Antimicrob. Chemother. 1:3-22.
Crossref

 
 

Firaol T, Sefinew A, Awoke T (2013). Aerobic microorganisms isolated from mastitic bovine milk and their antimicrobial susceptibility profiles in and around Gondar town, North Western Ethiopia. Am.-Euras. J. Agric. Environ. Sci. 13(7):920-925.

 
 

Fitzgerald JR, Sturdevant DE, Mackie SM, Gill SR, Musser JM (2001). Evolutionary genomics of Staphylococcus aureus: insights into the origin of methicillin-resistant strains and the toxic shock syndrome epidemic. Proc. Natl. Acad. Sci. USA 98(15):8821-6.
Crossref

 
 

Garedew L, Melese B, Tesfaye R (2015). Staphylococcus aureus in mastitic crossbreed cows and its associated risk factors in Addis Ababa City, Ethiopia. Ethiop. Vet. J. 19(1):107-116.
Crossref

 
 

Garipcin M, Seker E (2015). Nasal carriage of methicillin-resistant Staphylococcus aureus in cattle and farm workers in Turkey. Veterinarski Arhiv. 85(2):117-129.

 
 

Gebremichael L, Deressa B, Begna F, Mekuria A (2013). Study on prevalence of bovine mastitis in lactating cows and associated risk

 
 

Gebreyohanes T (2008). Prevalence of staphylococcus aureus mastitis associated milk loss and antimicrobial susceptibility of isolates of the pathogen in Debrezeit dairy farms. MSc. Thesis. Addis Ababa University, School of Agriculture and Veterinary Medicine, Debrezeit, Ethiopia.

 
 

Genigeorgis CA. (1989). Present state of knowledge on Staphylococcal intoxication. J. Food Microbiol. 9:327-360.
Crossref

 
 

Gianneechini R, Concha C, Franklin A (2002). Antimicrobial Susceptibility of Udder Pathogens Isolated from Dairy Herds in the West Littoral Region of Uruguay. Acta. Vet. Scand. 43:31-41.
Crossref

 
 

Gizat A. (2004): A cross-sectional study of bovine mastitis in and around Bahir Dar and antibiotic resistance patters of major pathogens. MSc. Thesis, Addis Ababa University, Faculty of Veterinary Medicine, Debre-zeit, Ethiopia.

 
 

Green M, Bradley A (2004). Clinical Forum -Staphylococcus aureus mastitis in cattle. Cattle_Practice Uk Vet. 9(4): 1-9

 
 

Groot MJ, van't Hooft E (2016). The Hidden Effects of Dairy Farming on Public and Environmental Health in the Netherlands, India, Ethiopia, and Uganda, Considering the Use of Antibiotics and Other Agro-chemicals. Front. Public Health. https://doi.org/10.3389/fpubh.2016.00012.
Crossref

 
 

Hawkey PM (2003). Mechanisms of quinolone action and microbial response. J. Antimicrob. Chemother. 51: 29-35.
Crossref

 
 

Huber H, Giezendanner N, Stephan R, Zweifel C (2011). Genotypes, antibiotic resistance profiles and microarray-based characterization of methicillin-resistant Staphylococcus aureus strains isolated from livestock and veterinarians in Switzerland. Zoonoses Public Health 58(5):343-349.
Crossref

 
 

Huber H, Koller, S., Giezendanner, N., Stephan, R. and Zweifel, C. (2009). Prevalence and characteristics of meticillin-resistant Staphylococcus aureus in humans in contact with farm animals, in livestock, and in food of animal origin, Switzerland. Eur. Surveill. 15:1-4.

 
 

ILRI (1996). International livestock research institute. Annual project report. ILRI, Addis Ababa, Ethiopia.

Jaims E, Montros L, Renata C (2002). Epidemiology of drug resistance; the case of Staphylococcus aureus and coagulase negative Staphylococci infections. Salud Publica Mex. 44(2):108-112.
Crossref

 

Jensen SO, Lyon BR (2009). Genetics of antimicrobial resistance in Staphylococcus aureus. Future Microbiol. 4(5):565-582.
Crossref

 
 

Kerro O, Tareke F (2003). Bovine mastitis in selected areas of Southern Ethiopia. Trop. Anim. Health Prod. 35:197-205.
Crossref

 
 

Kloos WE, Bannerman TL, Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RF (1995). Staphylococcus and Micrococcus. In: Manual of Clinical Microbiology. ASM Press, Washington, DC. pp. 282-298.

 
 

Kluytmans J (2010). Methicillin-resistant Staphylococcus aureus in food products: Cause for concern or case for complacency? Clin. Microbiol. Infect. 16(1):11-15.
Crossref

 
 

Kumar R, Yadav BR, Singh RS (2010). Genetic determinants of antibiotic resistance in Staphylococcus aureus isolates from milk of mastitic crossbred cattle. Curr. Microbiol. 60: 379-386.
Crossref

 
 

Kwon N, Park K, Jung W, Youn H, Lee Y, Kim S, Bae W, Lim J, Kim JY, Kim JM, Hong S, Park Y (2006). Characteristics of methicillin resistant Staphylococcus aureus isolated from chicken meat and hospitalized dogs in Korea and their epidemiological relatedness. Vet. Microbiol. 117(2-4):304-312.
Crossref

 
 

Land O'Lakes Inc. (2010). The next stage in dairy development for Ethiopia dairy value chains In End markets and food security cooperative agreement 663-A-00-05-00431-00. Land O'Lakes Inc., Addis Ababa, Ethiopia.

 
 

Leclercq R (2002). Staphylococcus resistant to antibiotic therapy. Annales Francaisesd' anaestheiseet de Reanimation 21:375-383.

 
 

Leloir Y, Baron F, Gautier M (2003). Staphylococcus aureus and food poisoning. Genet. Mol. Res. 2:63-76.

 
 

Levy S (1998). The challenge of antibiotic resistance. Sci. Am. 278:46-53.
Crossref

 
 

Loeffler A, Lloyd DH (2010). Companion animals: a reservoir for methicillin-resistant Staphylococcus aureus in the community? Epidemiol. Infect. 138:595-605.
Crossref

 
 

Lowy FD (1998). Staphylococcus aureus infections. N. Engl. J. Med. 339:520-532.
Crossref

 
 

Mekibib B, Furgasa M, Abunna F, Megersa B, Regassa A (2010). Bovine mastitis: Prevalence, risk factors and major pathogens in dairy farms of Holeta town, Central Ethiopia. Vet. World 13(9):397-403.
Crossref

 
 

Moges N, Yilikal A, Kelay B (2011). A cross sectional study on the prevalence of sub clinical mastitis and associated risk factors in and around Gondar, Northern Ethiopia. Int. J. Anim. Vet. Adv. 3(6):455-459.

 
 

Mulugeta Y, Wassie M (2013). Prevalence, risk factors and major bacterial causes of bovine mastitis in and around Wolaita Sodo, Southern Ethiopia. Glob. J. Microbiol. Res. http: //globalscienceresear chjournals.org/gjmr/645722013621.

 
 

NMC (2004). National mastitis council (NMC) Microbiological procedures for the diagnosis of bovine udder infection. National Mastitis Council Inc., 3rd ed, Madison, Wisconsin. pp. 7-8.

 
 

Normanno G (2005). Coagulase-positive staphylococci and Staphylococcus aureus in food products marketed in Italy. Int. J. Food Microbiol. 98:73-79.
Crossref

 
 

O'Mahony R, Abbott Y, Leonard FC, Markey BK, Quinn PJ, Pollock PJ, Fanning S, Rossney AS (2005). Methicillin-resistant Staphylococcusaureus (MRSA) isolated from animals and veterinary personnels in Ireland. Vet. Microbiol. 109:285-296.
Crossref

 
 

Otter A, French L (2010). Molecular epidemiology of community associated meticillin-resistant Staphylococcus aureus in Europe. Lancet Infect. Dis. 10(4):227-239.
Crossref

 
 

Pace JL, Yang G (2006). Glycopeptides. Update on an old successful antibioticclass. Biochem. Pharm. 71:968-980.
Crossref

 
 

Pantosti A, Sanchini A, Monaco M (2007). Mechanisms of antibiotic resistance in Staphylococcus aureus. Future Microbiol. 2(3):323-34.
Crossref

 
 

Quinn PJ, Carter ME, Markey BK, Carter GR (2004). Mastitis. In: Clinical Veterinary Microbiology, Mosby International Limited, London. pp. 327-344.

 
 

Radostits OM, Gay CC, Blood DC, Hinchllif KW (2007). Mastitis. In: Veterinary Medicine 9th ed., Haracourt Ltd, London. pp. 174-758.

 
 

Rall VLM, Miranda ES, Castilho IG, Camargo CH (2013). Diversity of Staphylococcus species and prevalence of enterotoxin genes isolated from milk of healthy cows and cows with subclinical mastitis. J. Dairy Sci. 97:829-837.
Crossref

 
 

Rota C, Yanguela J, Blanco D, Carraminana JJ, Arino A, Herrera A (1996). High prevalence of multiple resistances to antibiotics in 144 Listeria isolates from Spanish dairy and meat products. J. Food Prot. 59: 938-943.
Crossref

 
 

Rowe J. (1999). Milk quality and Mastitis. Small ruminant for mixed practitioner. Western Veterinary Conference, Lasvagas. pp. 152-156.

 
 

Sharma L, Verma AK, Kumar A, Rahat A, Neha, Nigam R (2015). Incidence and Pattern of Antibiotic Resistance of Staphylococcus aureus Isolated from Clinical and Subclinical Mastitis in Cattle and Buffaloes. Asian J. Anim. Sci. 9:100-109.
Crossref

 
 

Sisay G, Alo M, Ketema B, Teshale S, Fanos T, Tariku J (2012). Study on prevalence of bovine mastitis and its major causative agents in west Harerghe zone, Doba district, Ethiopia. J. Vet. Med. Anim. Health 4 (8):116-123.

 
 

Skov R, Smyth R, Clausen M, Larsen AR, Frimodt-Møller N, Olsson-Liljequist B (2003). Evaluation of a cefoxitin 30 μg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 52:204.
Crossref

 
 

Smith TC, Male MJ, Harper AL, Kroeger JS, Tinkler GP, Moritz ED, Capuano AW, Herwaldt LA, Diekema DJ (2009). Methicillin-resistant Staphylococcus aureus (MRSA) strain ST398 is present in midwestern U.S. swine and swine workers. PLoS ONE 4:4258.
Crossref

 
 

Sori T, Hussien J, Bitew M (2011). Prevalence and susceptibility assay of Staphylococcus aureus isolated from bovine mastitis in Dairy Farms of Jimma Town, South West Ethiopia. J. Anim. Vet. Adv. 10(6):745-749.
Crossref

 
 

Springer B, Orendi U, Much P, Höger G, Ruppitsch W, Krziwanek K, Metz-Gercek S, Mittermayer H (2009). Methicillin-resistant Staphylococcus aureus: a new zoonotic agent? Wiener klinische Wochenschrift. 121: 86-90.
Crossref

 
 

Swartz MN (2004). Human diseases caused by food borne pathogens of animal origin. Clin. Infect. Dis. 3:111-122.

 
 

Swenson JM, Brasso WB, Ferraro MJ, Hardy DJ, Knapp CC, Lonsway D, McAllister S, Reller LB, Sader HS, Shortridge D (2009). Correlation of cefoxitin MICs with the presence of mecA in Staphylococcus spp. J Clin Microbiol., 47: 1902-1905.
Crossref

 
 

Tassew A, Negash M, Demeke A, Feleke A, Tesfaye B, Sisay T (2016). Isolation, identification and drug resistance patterns of MRSA from mastitic cow's milk in and around Kombolcha, Ethiopia. J. Vet. Med. Anim. Health 8(1):1-10.
Crossref

 
 

Thrusfield M (2007). Sample size determination. In: Veterinary Epidemiology. 3rd edition, UK: Blackwell Science Ltd. pp. 185-189.

 
 

Ünal N, Askar Åž, Macun H, Sakarya F, Altun B, Yıldırım M (2012). Panton-valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants. Trop. Anim. Health Prod. 44:573-579.
Crossref

 
 

Van Loo I, Huijsdens X, Tiemersma E, De Neeling A, Van De Sande-Bruinsma N, Beaujean D, Voss A, Kluytmans J (2007). Emergence of methicillin-resistant Staphylococcus aureus of animal origin in humans. Emerg. Infect Dis. 13:1834-1839.
Crossref

 
 

Vanderhaeghen W, Hermans K, Haesebrouck F, Butaye P (2010). Methicillin-resistant Staphylococcusaureus (MRSA) in food production animals. Epidemiol. Infect. 138:606-625.
Crossref

 
 

Virgin JE, Van Slyke TM, Lombard JE, Zadoks RN (2009). Short communication: methicillin-resistant Staphylococcus aureus detection in US bulk tank milk. J. Dairy Sci. 92:4988-4991.
Crossref

 
 

Von Eiff C, Becker K, Machka K, Stammer H, Peters G (2001). Nasal carriage as a source of Staphylococcus au¬reus bacteremia. N Engl. J. Med. 344:11-16.
Crossref

 
 

Waage S, Bjorland J andCaugant DA (2002). Spread of Staphylococcus aureus resistant to penicillin and tetracycline within and between dairy herds. Epidemiol. Infect. 129:193-202.
Crossref

 
 

Wang S, Wu C, Shen J, Wu Y, Wang Y (2013). Hypermutable Staphylococcus aureus strains present at high frequency in subclinical bovine mastitis isolates are associated with the development of antibiotic resistance. Vet. Microbiol. 165:410-415.
Crossref

 
 

Workineh S, Bayleyeng M, Mekonnen H, Potgieter LND (2002). Prevalence and etiology of mastitis in cows from two major Ethiopian dairies. Trop. Anim. Health Prod. 34:19-25.
Crossref

 
 

Zadoks RN, Allore HG, Hagcnaars TJ, Barkema HW, Schukken YH (2002). A mathematical model of Staphylococcus aureus control in dairy herds. J. Epidemiol. Infect. 129:397-416.
Crossref

 
 

Zeryehun T, Aya T, Bayecha R (2013). Study on prevalence, bacterial pathogens and associated risk factors of bovine mastitis in smallholder dairy farms in and around Addis Ababa. Ethiopia. J. Anim. Plant Sci. 23(1):50-55.

 
 

Zutic M, Cirkovic I, Pavlovic L, Zutic J, Asanin J, Radanovic O, Pavlovic N (2012). Occurrence of methicillin-resistant Staphylococcus aureus in milk samples from Serbian cows with subclinical mastitis. Afr. J. Microbiol. Res. 6:5887-5889.