African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Comparative detection of African swine fever virus by loop-mediated isothermal amplification assay and polymerase chain reaction in domestic pigs in Uganda

David Kalenzi Atuhaire*
  • David Kalenzi Atuhaire*
  • 1. College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda; 2. National Agricultural Research Organization, National Livestock Resources Research Institute, P. O. BOX 96, Tororo, Uganda
  • Google Scholar
Mathias Afayoa
  • Mathias Afayoa
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar
Sylvester Ochwo
  • Sylvester Ochwo
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar
Dianah Katiti
  • Dianah Katiti
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar
Frank Norbert Mwiine
  • Frank Norbert Mwiine
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar
Ann Nanteza
  • Ann Nanteza
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar
Claire Mack Mugasa
  • Claire Mack Mugasa
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar
Enock Matovu
  • Enock Matovu
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar
Julius Boniface Okuni
  • Julius Boniface Okuni
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar
William Olaho-Mukani
  • William Olaho-Mukani
  • African Union-Interafrican Bureau of Animal Resources, P. O. BOX 30786, Nairobi, Kenya
  • Google Scholar
Lonzy Ojok*
  • Lonzy Ojok*
  • College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P. O. BOX 7062 Kampala, Uganda
  • Google Scholar


  •  Received: 22 April 2014
  •  Accepted: 19 May 2014
  •  Published: 04 June 2014

References

Agüero M, Fernández J, José Romero L, Zamora MJ, Sánchez C, Belák S, Arias M, Sánchez-Vizcaíno JM (2004). A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples. Vet. Res. 35(5):551-563.
Crossref
 
Atuhaire KD, Afayoa M, Ochwo S, Mwesigwa S, Mwiine FN, Okuni JB, Olaho-Mukani W, Ojok L (2013). Prevalence of African swine fever virus in apparently healthy domestic pigs in Uganda. BMC Vet. Res. 9:263-271.
Crossref
 
Atuhaire KD, Ochwo S, Afayoa M, Mwiine FN, Ikwap K, Arinaitwe E, Ademun OR, Okuni JB, Nanteza A, Ayebazibwe C, Okedi L, Okuni JB, Olaho-Mukani W, Ojok L (2013). Epidemiological overview of African swine fever in Uganda (2001-2012). J. Vet. Med. 949638:9.
 
Björnheden L (2011). A study of domestic pigs, wild suids and ticks as reservoirs for African swine fever virus in Uganda. MSc.Thesis. Sveriges lantbruksuniversitet. Retrieved from http://epsilon.slu.se
 
Costard S, Wieland B, De Glanville W, Jori F, Rowlands R, Vosloo W, Roger F, Pfeiffer DU, Dixon LK (2009). African swine fever: how can global spread be prevented? Philosophical transactions of the Royal Society of London Series B. Biol. Sci. 364:2683-2696.
Crossref
 
Dixon LK, Costa JV, Escribano JM, Kock DL, Vi-uela E, Wilkinson PJ (2000). Family Asfarviridae. In Van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK (eds). Virus taxonomy, 7th Report of the ICTV, Academic Press, San Diego, pp.159-165.
 
Gallardo C, Ademun AR, Nieto R, Nantima N, Arias M, Pelayo V, Bishop RP (2011). Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007. Afr. J. Biotechnol. 10(17):3488-3497.
 
Gallardo C, Mwaengo D, Macharia J (2009). Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes. Virus Genes, 38:85-95.
Crossref
 
Giammarioli M, Pellegrini C, Casciari C, De Mia GM (2008). Development of a novel hot-start multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus. Vet. Res. Commun. 32(3):255-262.
Crossref
 
Hjertner B, Meehan B, Mchkillen J, McNeilly F, Belák S (2005). Adaptation of an Invader® assay for the detection of African swine fever virus DNA. J.Virol. Methods 1-2:1-10.
Crossref
 
James HE, Ebert K, McGonigle R, Reid SM, Boonham N, Tomlinson JA, Hutchings GH, Denyer M, Oura CAL, Dukes JP, King DP (2010). Detection of African swine fever virus by loop-mediated isothermal amplification. J. Virol. Methods 164(1-2):68-74.
Crossref
 
Khan GM, Rifat K, Bhaskar H, Salam A, Akther T (2012). Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of Leishmania DNA in buffy coat from visceral leishmaniasis patients. Parasi. Vectors, 5(1):280-288.
Crossref
 
King DP, Reid SM, Hutchings GH, Grierson SS, Wilkinson PJ, Dixon LK, Bastos ADS, Drew TW (2003). Development of a TaqMan PCR assay with internal amplification control for the detection of African swine fever virus. J. Virol. Methods, 107(1):53-61.
Crossref
 
Koizumi N, Nakajima C, Harunari T, Tanikawa T, Tokiwa T, Uchimura E, Furuya T (2012). A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira species in Urine. J. Clin. Microbiol. 50(6):2072-2074.
Crossref
 
Malmquist W, Hay D (1960). Haemadsorption and cytopathic effect produced by African swine fever virus in swine bone marrow and buffy coat cultures. Am. J. Vet. Res. 21:104-108.
Pubmed
 
McKillen J, McMenamy M, Hjertner B, McNeilly F, Uttenthal A, Gallardo C, Adair B, Allan G (2010). Sensitive detection of African swine fever virus using real-time PCR with a 5' conjugated minor groove binder probe. J. Virol. Methods, 168(1-2):141-146.
Crossref
 
Mckinnon A (2000). A spreadsheet for the calculation of comprehensive statistics for the assessment of diagnostic tests and inter-rater agreement. Comp Biol. Med. 30(3):127-134.
Crossref
 
Nagamine K, Hase T, Notomi T (2002). Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell. Probe. 16:223-229.
Crossref
 
Nakao R, Stromdahl EY, Magona JW, Faburay B, Namangala B, Malele I, Inoue N, Geysen D, Kajino K, Jongejan F, Sugimoto C (2010). Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Detection of Ehrlichia ruminantium. BMC Microbiol. 10(1):296-307.
Crossref
 
Namangala B, Hachaambwa L, Kajino K, Mweene AS, Hayashida K, Simuunza M, Simukoko H, Choongo K, Chansa P, Lakhi S, Moonga L, Chota A, Ndebe J, Nsakashalo-Senkwe M, Elizabeth Chizema E, Kasonka L, Sugimoto C (2012). The use of Loop-mediated Isothermal Amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi valleys. Parasi. Vectors, 5(1):282-287.
Crossref
 
Njiru Z, Mikosza A, Armstrong T, Enyaru J, Ndung'u J, Thompson A (2008). Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense. PLoS Negl. Trop. Dis. 2(2):e147.
Crossref
 
Njiru ZK, Ouma JO, Bateta R, Njeru SE, Ndungu K, Gitonga PK, Guya S, Traub R (2011). Loop-mediated isothermal amplification test for Trypanosoma vivax based on satellite repeat DNA. Vet.Parasitol. 180(3-4):358-362.
Crossref
 
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28(12):E63.
Crossref
 
Office International des Epizooties (OIE) (2010). Manual of diagnostic tests and vaccines for terrestrial animals.
 
Oura CA, Powell P, Parkhouse RM (1998). Detection of African swine fever virus in infected pig tissues by immunocytochemistry and in situ hybridisation. J. Virol. Methods, 72(2):205-217.
Crossref
 
Ronish B, Hakhverdyan M, Ståhl K, Gallardo C, Fernandez-Pinero J, Belák S, Leblanc N, Wangh L (2011). Design and verification of a highly reliable Linear-After-The-Exponential PCR (LATE-PCR) assay for the detection of African swine fever virus. J. Virol. Methods, 172(1-2):8-15.
Crossref
 
Rutebarika C, Ademun AO (2011). Overview of African Swine Fever (ASF) Impact and surveillance in Uganda. During African Swine Fever Diagnostics, Surveillance, Epidemiology and Control: Identification of Researchable Issues Targeted to the Endemic Areas within sub-Saharan Africa. Nairobi, Kenya.
 
Steiger Y, Ackermann M, Mettraux C, Kihm U (1992). Rapid and biologically safe diagnosis of African swine fever virus infection by using polymerase chain reaction. J. Clin. Microbiol. 30:1-8.
Pubmed
 
Tejlar E (2012). Outbreaks of African swine fever in domestic pigs in Gulu district, Uganda. MSc. Thesis. Retrieved from http://stud.epsilon.slu.se/4081/1/tejler_e_120430.pdf
 
Tie Z, Chunguang W, Xiaoyuan W, Xinghua Z, Xiuhui Z (2012). Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus in Dairy Cow Suffering from Mastitis, J. Biomed. Biotechnol., 2012, Article ID 435982, p.5.
 
Uganda Beaural of Statistics/The Ministry Of Agriculture, Animal Industry and Fisheries. (2009). The National Livestock Census Report 2008. Kampala, Uganda.
 
Wilkinson PJ (2000). African swine fever. In Manual of standards for diagnostic test and vaccines, Office International des Epizooties. Paris, France, (4th ed), pp.189-198.
Pubmed
 
Yamazaki W, Seto K, Taguchi M, Ishibashi M, Inoue K (2008). Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loopmediated isothermal amplification. BMC Microbiol. 8:94-101.
Crossref
 
Zhou S, Han S, Shi J, Wu J, Yuan X, Cong X, Xu S, Wu X, Li J, Wang J (2011). Loop-mediated isothermal amplification for detection of porcine circovirus type 2. Virol J. 8(1):497-502.
Crossref
 
Zsak L, Borca MV, Risatti GR, Zsak A, French RA, Lu Z, Kutish GF, Neilan JG, Callahan JD, Nelson WM, Rock LD (2005). Preclinical Diagnosis of African Swine Fever in Contact-Exposed Swine by a Real-Time PCR Assay. J. Clin. Microbiol. 43(1):112-119.
Crossref