March 2014
Plant growth promoting rhizobacteria (PGPR) induces resistance against Fusarium wilt and improves lycopene content and texture in tomato
Plant growth promoting Bacillus subtilis (BS2) was found effective against tomato wilt caused by Fusarium oxysporum f sp. lycopersici under field conditions. Pretreatment of tomato plants with B. subtilis BS2 significantly induced the activities of defense related enzymes viz., peroxidase, polyphenol oxidase, chitinase and phenylalanine ammonialyase and phenolics when challenged with the pathogen. Apart from disease...
March 2014
Assessment of five phenotypic tests for detection of methicillin-resistant staphylococci in Cotonou, Benin
The study aimed at assessing performance and cost of phenotypic tests for detecting methicillin resistance in Staphylococcus spp. isolates in Cotonou, Benin. Isolates consecutively collected from various specimens from four medical laboratories in Cotonou from December 2012 to April 2013 were included in the study. The isolates were subjected to five phenotypic tests: disk diffusion tests with cefoxitin (Cefox) and...
March 2014
Bacteria from infected surgical wounds and their antimicrobial resistance in Hawassa University Referral Teaching Hospital, Southern Ethiopia
A study was carried out from November 2010 to June 2011 in Hawassa University Referral Teaching Hospital to identify bacterial species involved in post-operative wound infections and to determine their antimicrobial resistance pattern. The study involves 100 surgical patients with post-surgical wound infections. Swab samples of wound discharge were collected for bacteriological examination and inoculated on appropriate...
March 2014
Qualitative and quantitative study on bacterial flora of farm raised common carp, Cyprinus carpio in India
Analysis of pond water and sediment as well as skin and intestine of common carp, cultured under polycultre system, was done quantitatively and qualitatively. During the study of 60 days (winter and summer seasons), total viable counts of bacteria were in the range of 4.43±0.50x103 to 5.5 ±0.09x103 cfu g-1 and 7.43±0.03x103 to 9.66±0.09x103 cfu g-1, respectively in water of A, B and C ponds....
March 2014
Salmonella spp. detection in chicken meat and cross-contamination in an industrial kitchen
Chicken meat is a widely consumed food. However, broilers are implicated in contamination by Salmonella spp., since poultry is considered asymptomatic carrier of the pathogen. The objective of this study was to detect the presence of Salmonella spp. in chicken in natura and ready for consumption, as well as in the hands of employees, personal protection equipment and utensils in an industrial kitchen. In total, 18 in...
March 2014
Characterization of nematode community in apple orchard soil in the Northwestern Loess Plateau, China
This work was carried out in the Northwestern Loess Plateau (NLP), one of two main large-scale apple production areas in China. It is to investigate the role of nematodes in apple production areas, which cause apple replant disease (ARD). Soil samples from total eight sites in NLP were collected twice, in summer and autumn, respectively. The nematodes were extracted by washing-sifting-sucrose centrifugation and total...
March 2014
Bio-detoxification of Jatropha curcas seed cake by Pleurotus ostreatus
The detoxification of Jatropha curcas seed cake is of major interest for the biodiesel industry to add economic value to this residue and also to reduce the environmental damage caused by its inappropriate disposal. In this context, the treatment of this residue with white rot fungus, Pleurotus ostreatus, can be a viable alternative because it produces enzymes capable of degrading different lignocellulosic residues and...
March 2014
Phytochemical analysis and antimicrobial bioactivity of the Algerian parsley essential oil (Petroselinum crispum)
In this paper, we extracted, analyzed and studied the antimicrobial activity of Algerian parsley essential oil on several microbes that cause infectious diseases and its effects on kinetics of lactic acid production by Lactobacillus casei subsp. rhamnosus. The essential oil of parsley (Petroselinum crispum Hoffm) obtained by hydrodistillation was characterized by its physicochemical properties and by its chromatographic...
March 2014
Assessment of detoxification of malathion by Pseudomonas isolates
Malathion degrading bacteria were isolated from a soil sample. Growth of mono and co-cultured strains of Pseudomonas in media containing the organophosphate insecticide, malathion as sole source of carbon was employed to assess degradability of the bacteria. On the seventh day of inoculation, bacteria free culture fluids as well as un-inoculated fluids were analyzed by gas chromatography and mass spectrometry (GC-MS)....
March 2014
Isolation, characterization and antimicrobial activity of a Streptomyces strain isolated from deteriorated wood
Emergence of drug resistance among pathogenic bacteria to currently used antibiotics has made the search for novel bioactive compounds from natural and unexplored habitats a necessity. In this study, we reported the isolation, characterization and antimicrobial activity of an actinomycete strain isolated from deteriorated wood of an old house located in the Medina of Fez. The isolate, named H2, was identified by 16S...
March 2014
Selection and characterization of Clostridium bifermentans strains from natural environment capable of producing 1,3-propanediol under microaerophilic conditions
In this work, we aimed to select from natural environment non-pathogenic strains of Clostridium spp. capable of producing 1,3-propanediol (1,3-PD). As a result we isolated 2256 of Clostridium spp., including 10 strains from the genus Clostridium bifermentans. It occurred that all isolates of this species were able to synthesis 1,3-PD on the level of ca. 10 g/L. Additionally, they synthetized metabolites in...
March 2014
Multiple-locus variable-number tandem-repeat analysis of Bacillus anthracis isolated from a human-animal anthrax outbreak in the Luangwa valley of Zambia
The incidence of anthrax, caused by Bacillus anthracis, in human and animal population of Zambia has increased recently. In this study, 34 strains of Bacillus anthracis from soil, hippopotamuses and humans, isolated in the 2011 outbreak were analyzed using the multiple-locus variable-number tandem repeat analysis. The analysis revealed that a single anthrax clone may have been involved in the epidemic. Considering the...
March 2014
First report of isolation and identification of Brevundimonase (Pseudomonas) diminuta from collected nasopharyngeal specimens in suspected patients to pertussis
Brevundimonase diminuta as an opportunistic environmental bacterium, due to the increase in isolation rate from clinical specimens and its antibiotic resistance is considered as a new threat to human health care. After performing conventional phenotypic methods and molecular approaches, antibiotics resistance pattern on the identified Brevundimonase isolates was performed as minimum bactericidal concentration by disk...
March 2014
Mathematical modeling of some medium constituents and its impact on the production of vitamin B12 and folic acid by Klebsiella pneumoniae under solid state fermentation
The present investigation involved testing the effect of different carbon sources (glucose, sucrose and starch) as well as different nitrogen sources (peptone, yeast extract and urea) on the production of vitamin B12 using Klebsiella pneumonia grown on mixture of agriculture wastes (wheat straw and rice bran). The results show that glucose was the best carbon source compared to the other di- and polysaccharides used....
March 2014
Influence of some chemical parameters on decolorization of textile dyes by bacterial strains isolated from waste water treatment plant
Bacterial species capable of decolorizing textile and laboratory dyes were isolated from textile effluent treatment plant. Two bacterial strains (one Gram negative and another Gram positive bacterial strain) were screened for their ability to decolorize Red H5BL (reactive dye), Thymol blue (acid dye), Malachite green, Crystal violet (Triphenyl dye) and Congo red (azo dye). The highest decolorization was achieved for Red...
March 2014
First characterization of CTX-M-15 and DHA-1 β-lactamases among clinical isolates of Klebsiella pneumoniae in Laghouat Hospital, Algeria
Extended-spectrum β-lactamases (ESBL) are a problem of great concern owing to the potential transmission of resistance to others bacterial species. Our study aims to investigate the ESBL and plasmid-mediated β-lactamases produced by Klebsiella pneumoniae strains isolated from 2010 to 2012, among patients hospitalized in "Ahemida Ben Adjila" hospital, Laghouat, Algeria, and to seek a possible clonal...
March 2014
Postharvest fruit spoilage bacteria and fungi associated with date palm (Phoenix dactylifera L) from Saudi Arabia
Date fruits are consumed as traditional and ideal food in Saudi Arabia. It provides a wide range of essential nutrients and potential health benefits. Twelve (12) most consumed varieties of date fruits and their seeds available in the open markets of Riyadh, Medina and Kharj were screened for the presence of bacteria and fungi. Our study reveals that these fruits carry a heavy load of both fungal and bacterial...
March 2014
Efficacy of Mannheimia haemolytica A2, A7, and A2 and A7 combined expressing iron regulated outer membrane protein as a vaccine against intratracheal challenge exposure in sheep
This experimental study was done on a total of 40 male lambs with the objectives of developing experimental vaccines from Mannheimia haemolytica serotypes A2 and A7 that express iron regulated outer membrane protein and in vivo evaluation of their efficacy. Lambs were categorized in to four experimental groups and vaccinated with 1 ml of vaccine containing 5 × 108 CFU/ml. Group 1 was vaccinated with M. haemolytica...
March 2014
Differential immunoreactivity of the root-knot nematodes, Meloidogyne graminicola and Meloidogyne incognita to polyclonal and monoclonal antibodies and identification of antigens through proteomics approach
Meloidogyne graminicola infect graminaceous plants but have lesser tendency to infect dicotyledonous plants. Meloidogyne incognita is a pest of dicots and occasionally infects cereals. Evolutionary adaptation of these root-knot nematodes to their preferred hosts might have led to variability in their gene/protein profile which could contribute to their differential behaviour outside and inside the different host crops....
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