African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

First characterization of CTX-M-15 and DHA-1 β-lactamases among clinical isolates of Klebsiella pneumoniae in Laghouat Hospital, Algeria

Nouria Lagha*
  • Nouria Lagha*
  • 1. Laboratoire de microbiologie Appliquée à l’Agroalimentaire Au Biomédical et à l’Environnement « LAMAABE », faculté SNV-STU, université de Abou Bekr Belkaïd, B.P 119, Tlemcen 13000, Algérie; 2. Laboratoire de microbiologie, faculté des sciences biologiques, université Amar Telidji, Laghouat 03000, Algérie
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Djamel Eddine Abdelouahid
  • Djamel Eddine Abdelouahid
  • Laboratoire de microbiologie Appliquée à l’Agroalimentaire Au Biomédical et à l’Environnement « LAMAABE », faculté SNV-STU, université de Abou Bekr Belkaïd, B.P 119, Tlemcen 13000, Algérie
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Hafida Hassaine
  • Hafida Hassaine
  • Laboratoire de microbiologie Appliquée à l’Agroalimentaire Au Biomédical et à l’Environnement « LAMAABE », faculté SNV-STU, université de Abou Bekr Belkaïd, B.P 119, Tlemcen 13000, Algérie
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Fréderic Robin3
  • Fréderic Robin3
  • 3Laboratoire de Bactériologie-Mycologie-Parasitologie, Hôpital Gabriel-Montpied ; 58 rue Montalembert 63003 Clermont-Ferrand, Cédex. France
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Richard Bonnet
  • Richard Bonnet
  • 3Laboratoire de Bactériologie-Mycologie-Parasitologie, Hôpital Gabriel-Montpied ; 58 rue Montalembert 63003 Clermont-Ferrand, Cédex. France
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  •  Received: 31 July 2013
  •  Accepted: 17 February 2014
  •  Published: 12 March 2014

Abstract

Extended-spectrum β-lactamases (ESBL) are a problem of great concern owing to the potential transmission of resistance to others bacterial species. Our study aims to investigate the ESBL and plasmid-mediated β-lactamases produced by Klebsiella pneumoniae strains isolated from 2010 to 2012, among patients hospitalized in "Ahemida Ben Adjila" hospital, Laghouat, Algeria, and to seek a possible clonal dissemination. Antimicrobial susceptibility testing was performed by the agar diffusion method, the characterization of resistance genes to β-lactam antibiotics was performed by PCR amplification and gene sequencing, and molecular typing was performed by ERIC-PCR. In total, of 112 clinical strains of K. pneumoniae isolated, nine isolates produced an ESBL. Antibiotics susceptibility testing showed a complete resistance to the majority of third-generation cephalosporins and a very frequent resistance to aminoglycosides and fluoroquinolones resistance. PCR analysis and sequencing showed that all isolates produced ESBL CTX-M-15; three strains of them also produced the cephalosporinase DHA-1. Molecular typing showed that most strains were not related; only three strains that produced CTX-M-15 and DHA-1 had identical profiles suggesting a clonality link. This study revealed the dissemination of ESBL CTX-M-15 and plasmid-mediated AmpC cephalosporinase DHA-1 in K. pneumoniae. This is a first report of these enzymes at the Laghouat hospital.

 

Key words: Klebsiella pneumoniae, resistance, CTX-M-15, DHA-1, genotyping, Algeria.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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