November 2012
Traditional Chinese medicine for the treatment of psoriasis vulgaris: A systematic review
With a long history, the treatment of psoriasis by traditional Chinese medicine (TCM) could be divided into prophase, metaphase and anaphase. The earliest description of psoriasis dated back to A.D. 581 in ancient Chinese Song Dynasty. After that, a large number of treatment methods emerged with the increasing understanding of the disease. In this paper, a statistical analysis of the TCM category and frequency for the...
November 2012
Bacterial diversity as affected by tillage practice in a raised bed planting system
Carbon and nitrogen dynamics are altered by tillage so it was hypothesized that the composition of the microbial population that control these processes was affected too. Soil was sampled from conventional beds (CB) tilled and reformed each year with crop residue incorporation and permanent raised beds (PB) with zero tillage seeding and residue retention on the top soil, while the bacterial community...
November 2012
Influence of the seasonal conditions on phenolic composition and antioxidant activity of Vitis vinifera L.
The aim of this study is determining the phenolic acid composition and antioxidant activity potential of Vitis vinifera collected from Osmaniye-Turkey. For determining the phenolic acid composition of the grapes collected in different seasons, samples were screened via a high performance liquid chromatography-diode array detection (HPLC-DAD) instrument. By this way, amounts of gallic acid,...
November 2012
Simple and rapid protocol for isolation of chromosomal and plasmid DNA from Saccharomyces cerevisiae suitable for polymerase chain reaction analysis
Majority of the shuttle vectors developed for the metabolic engineering of yeast have low copy number, hence the need to be rescued and optimized in Escherichia coli prior to genetic manipulation studies. Methods that are currently available are complicated, contain lot of detergents, time consuming and often yield poor quality plasmid DNA. The present method describes a simple and rapid protocol for isolation...
November 2012
A preliminary survey on the ticks carrying Ehrlichia and Anaplasma in the Southern marginal zone of Gurbantunggut Desert
To understand the status of ticks carrying Ehrlichia and Anaplasma in the southern marginal zone of Gurbantunggut Desert, we collected and classified ticks from livestock in 11 locations of three different habitats in the southern marginal zone of Gurbantunggut Desert. Ticks were screened by nested polymerase chain reaction (PCR) and their 5' hypervariable regions of 16S rRNA genes were amplified...
November 2012
Investigation of in vitro effects of estrogens and selective estrogen receptor modulators (serms) on Trichomonas vaginalis
The aim of this study was to investigate if there is a relationship between the hormones and Trichomonas vaginalis growth. A strain of T. vaginalis, was incubated and cultivated to compare the in vitro effects of 17β-Estradiol, Estriol, Raloxifene, Tamoxifen on T. vaginalis trophozoites in terms of different concentrations. High concentrations of 17β-Estradiol...
November 2012
Immunogenetic regulation of HLA-DR, DQ, DP and CD1a positive cells in the pathogenesis of keloid and hypertrophic scar
Recent studies have shown that HLA-DR, DQ, DP and CD1a positive cells were related to keloid and hypertrophic scar. Therefore, this study was aimed at investigating the role of immunogenetic factors in the pathogenesis of keloid and hypertrophic scar. The expression levels and distribution patterns of HLA-DR, -DQ, -DP and CD1a molecules in peripheral blood mononuclear cells were determined in 10 samples of keloid,...
November 2012
Systematic study on comparing phytochemicals and the antimicrobial activities from different parts of V. amygdalina
The leaf, stem and root bark of Vernonia amygdalina were subjected to phytochemical screening for the presence of alkaloids, saponins, cardiac glycosides, flavonoids, anthraquinones, steroids, polyphenols, and phlobatannins. The root bark possessed all the phytochemical components tested. Stem bark possessed all the phytochemical components except flavonoids, while the leaf lacks anthraquinones and...
November 2012
Impact of different polymerase chain reaction (PCR) strategies on denaturing gradient gel electrophoresis-based analysis of bacterial communities in soils/sediments from the Northern Jiangsu Oil Field, China
The selection of polymerase chain reaction (PCR) strategies is vital to a successful assessment of bacterial communities in soils/sediments by 16S rRNA gene-based denaturing gradient gel electrophoresis (DGGE) analysis. To obtain reliable information of the bacteria communities in soils/sediments from the...
November 2012
Efficient expression of TdiA, a single-module nonribosomal peptide synthetase in Escherichia coli Rosetta (DE3) for enzymatic synthesis of bis-indolylquinone
TdiA, a nonribosomal peptide synthetase, catalyzes the carbon-carbon bond formation in biosynthesis of bis-indolylquinone natural products. The TdiA gene (tdiA) containing several codons rarely used by Escherichia coli, was cloned from the genome ofAspergillus nidulans and optimized in two strains of E. coli: BL21 (DE3) and Rosetta (DE3), which is a rare codon optimizer strain. The effects of initial...
November 2012
Characterization and genotyping by pulsed-field gel electrophoresis (PFGE) of the first bioluminescent Vibrio gigantis strains
The genus Vibrio, which included 20 species in 1981, increased up to 63 in 2004 and in the last 10 years, 15 new species including Vibrio gigantis have been added. In this study, 20 bioluminescent bacteria were isolated and identified. The identification of the species whose various physiological and biochemical characteristics were identified was based on 16S rRNA gene region and it was determined that...
November 2012
Spa type of Erysipelothrix strains and its association with virulence of Erysipelothrix strains in mice and swine
We investigated the prevalence of spaA, spaB and spaC in 90 virulent or avirulentErysipelothrix strains. All Erysipelothrix rhusiopathiae strains (n = 58) harboured eitherspaA or spaB, whereas all other sp.-2 (n = 3) strains harboured spaC. None of the other species, including Erysipelothrix tonsillarum (n = 25) and other sp.-1 (n = 2) and sp.-3 (n =...
November 2012
High throat carriage of Groups G/C streptococci among school children in Bangalore City and its significance
The present study was designed to determine the prevalence of comparative throat carriage of diverse antigenic groups of beta hemolytic streptococci (BHS) among school children. BHS were identified by Lancefield grouping with commercial kits. BHS were isolated from 112 (10.4%) of 1156 asymptomatic children, of which 36 (32.1%) belonged to Group A, 55 (49.1%) to Group G and 11 (9.8%) to Group C. A throat carrier rate of...
November 2012
Methicillin-resistance Staphylococcus aureus detection by an improved rapid polymerase chain reaction (PCR) assay
In this study, a novel orfX-PCR for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) had been developed and applied, with the specific targeting orfX on SCCmec. PCR amplification generated one specific band with size of 212 bp, and high specificity was obtained. Application of this PCR had been further performed on detection for 262 MRSA and MRCNS strains,...
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