African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Methicillin-resistance Staphylococcus aureus detection by an improved rapid polymerase chain reaction (PCR) assay

Rong You1,2, Zhongyu Gui1, Zhenbo Xu1,3*, Mark E. Shirtliff3, Guangchao Yu4, Xihong Zhao1,5,6, Lei Shi1, Bing Li1, Jianyu Su1 and Lin Li1*
1College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China. 2Department of Microbial Pathogenesis, Dental School, University of Maryland, Baltimore, MD 21201, USA. 3First Affiliated Hospital of Jinan University, Guangzhou 510620, China. 4Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China. 5Department of Food Science and Biotechnology and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Gangwon 200-701, South Korea. 6South China Sea Fisheries Research Institute, Guangzhou 510300, China.
Email: [email protected]

  •  Accepted: 23 May 2012
  •  Published: 13 November 2012

Abstract

In this study, a novel orfX-PCR for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) had been developed and applied, with the specific targeting orfX on SCCmec. PCR amplification generated one specific band with size of 212 bp, and high specificity was obtained. Application of this PCR had been further performed on detection for 262 MRSA and MRCNS strains, and orfX-PCR results showed expected products for either MRSA or MRCNS strains, demonstrating the orfX-PCR assay to be a useful and powerful MRSA detection method.

 

Key words: Staphylococcus, MRSA, orfX-PCR, rapid detection.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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