April 2011
An overview of honey: Therapeutic properties and contribution in nutrition and human health
Honey, a natural product of very high nutritive value is made when the nectar (floral) and sweet deposits from plants (non floral) are gathered, modified and stored in the honeycombs by honeybees of the genera Apis and Meliponini. Its composition and quality vary greatly with the botanical source of nectar as well as environmental and climatic conditions. Depending on its quality, honey...
April 2011
Clarithromycin resistance and 23S rRNA mutations in Helicobacter pylori isolates in Iran
To determine the 23S rRNA point mutations in clarithromycin resistance ofHelicobacter pylori strains isolated from southwest, Iran. This was a cross-sectional survey, which was done on 263 patients who referred to endoscopy department of Shehrekord university of medical sciences. According to gram stain, urease, catalase, oxidase and polymerase chain reaction (PCR) H....
April 2011
Feasibility of using dot blot hybridization to detect Salmonella InvA, SpiC and SipC directly from clinical specimens
Pathogenesis of Salmonella depends upon a large number of factors controlled by an array of genes that synergise into actual virulence. The goal of this study was to detectSalmonella invA, spiC and sipC directly from clinical specimens, using the dot blot hybridization assay. We detected invA, spiC and sipC as a one combination from 4.5% (95% CI:...
April 2011
Prevalence of Mycoplasma hominis and Ureaplasma urealyticum among women with unexplained infertility, with and without vaginitis and cervicitis
The main objective of this study was to find the incidence of urogenital mycoplasma such asMycoplasma hominis and Ureaplasma urealyticum among patients with history of infertility of unexplained origin and to ascertain their implications in non specific cervicitis and non specific vaginitis. The total number of (n = 337) female patients with history of un-explained vaginitis and cervicitis were...
April 2011
Saccharomyces cerevisiae strains with robust responses to fermentation stresses isolated from the alcoholic fermentation of Agave duranguensis musts
Yeasts used in fermentation processes are subject to different stress conditions, including low pH, high initial substrate concentration, accumulation of toxic compounds (e.g. ethanol), and temperature fluctuations. The fermentation of mescal is conducted under variable environmental conditions throughout the year. One of the most important environmental factors is temperature, as autumn and winter...
April 2011
Spatial dynamics in physicochemistry and bacterio- and myco-plankton assemblages of Imo River in a Niger Delta community in Nigeria
We investigated the spatial relationships between physicochemical variables and planktonic (bacterio- and myco-plankton) assemblages of the middle reaches of the Imo River. In situmeasurements and collection of water samples for laboratory analyses of physicochemical characteristics and enumeration of microbial groups were made at 7 sampling locations along the river between March 2007 and February 2008...
April 2011
Investigation of Entamoeba histolytica and Mycobacterium spp. in biopsy specimens of patients with inflammatory bowel disease in Turkey
Intestinal amebiasis and gastrointestinal tuberculosis can mimic inflammatory bowel disease and its exacerbations clinically, pathologically, radiologically and endoscopically. In the existence of IBD and/or either one of these two pathogens, early identification and prompt treatment can improve the clinical course of the patients. The aim of this study was to investigate the presence of Entamoeba...
April 2011
Microbiological assessment of mutton intended for export from Elkadaro export slaughter house, Sudan
The study is planned to evaluate the hygienic quality of mutton intended for export from Elkadaro slaughter house on basis of surface bacterial contamination. Seventy five samples were collected during five visits, 15 samples in each visit (five samples for each: slaughter hall, slaughter house chiller and the refrigerated vehicle at Airport after unloading). A metallic triangle was used as a template from...
April 2011
Antibacterial activity of several Malaysian leaves extracts on the spoilage bacteria of yellow alkaline noodles
Yellow alkaline noodle (YAN) is very susceptible to spoilage and has a short shelf life due to the high moisture content. This study was conducted to isolate and identify spoilage bacteria of YAN in an attempt to apply local plant extracts that possess antibacterial activity in extending the shelf life of YAN. Thirty colonies were isolated from spoiled YAN and were identified using the Biolog GEN III system....
April 2011
Outer membrane proteins and morphological alterations of Shigella spp. under starvation in seawater
In this study, we incubated four strains of Shigella in seawater microcosms (at room temperature and at 4°C) for eight months and we studied the alteration of their morphologic and outer membranes proteins. The starved cells showed an evolution to the filterable minicells state capable to pass membrane pore size 0.45 µm. In addition, the atomic force micrographs showed a reduction of...
April 2011
New hosts of 16SrI phytoplasma group associated with edible Opuntia ficus-indica crop and its pests in Mexico
In Mexico in the region of Nopaltepec in Mexico State, the edible Cactus crop, Opuntia ficus-indica is mainly cultivated for prickly pear fruit production. This crop has problems with common pests (insects, mollusks and weeds) which may serve as reservoirs and together with the named Cactus with phytoplasma-like symptoms which through the time inhibit the fruit production, and for this reason, the...
April 2011
Interaction of Mycobacterium tuberculosis ESAT-6 protein with ADAM9 protein
The ESAT-6 protein of Mycobacterium tuberculosis (M. tb) is an important structural and functional protein, which has been known to be involved in the virulence, pathogenesis as well as proliferation of the pathogen; however, how ESAT-6 protein interact with host protein is still unclear. In order to study the function of the M. tuberculosis protein ESAT-6 in the...
April 2011
Production and oil-emulsion formulation of Cadophora malorum and Alternaria jacinthicola, two biocontrol agents against Water Hyacinth (Eichhornia crassipes)
Cadophora malorum isolate Mln715 and Alternaria jacinthicola strain MUCL 53159 are under development as biocontrol agents against Water Hyacinth (Eichhornia crassipes) in Mali. Production of spores of these agents on locally available substrates (Water Hyacinth, powdered paddy rice chaff, wheat semolina) was assessed with a view to mass production. The C. malorum isolate...
April 2011
Production, purification and characterization of α-amylase from Trichoderma harzianum grown on mandarin peel
The production, purification and characterization of α-amylase from Trichodermaharzianum grown on mandarin peel were investigated. The effect of incubation time and mandarin peel concentration on the production of α-amylase by T. harzianum was studied. α-Amylase A3 was purified from T. harzianum to electrophoretic homogeneity by using DEAE-Sepharose and...
April 2011
NFκB is differentially activated in macrophages from J774A.1 cell line infected with vaccine or virulent strains of Brucella abortus
The activation and nuclear translocation of NF-kB and the expression of the pro-inflammatory cytokine genes by macrophages infected with the attenuated Brucella abortus RB51 and virulent 2308 strains were evaluated. pIkBα and NF-kB were determined by immunoblot, and cytokines IFN-g and IL12 mRNA were determined by reverse transcriptase polymerase chain reaction (qPCR) and translocation of...
April 2011
Xylanase activity from Cellulomonas flavigena extracts as affected by temperature and its degradation under in vitro ruminal conditions
The objective of this study was to evaluate the enzyme extracts from Cellulomonas flavigena cultured at different temperatures using sugarcane bagasse as substrate to determine the xylanolytic activity and the degradation of the enzyme extracts protein underin vitro rumen conditions. Three enzyme extracts were obtained by liquid fermentation at 30, 37 and 40°C from C....
April 2011
Tissue inflammatory response and salivary Streptococcus mutans count with three different denture cleansers
This study was conducted to detect the clinical effect on mucosa and the bacteriological effect on saliva of three types of commercially available denture cleansers. Forty edentulous patients free from any oral or systemic diseases were selected in this study. Conventionally constructed dentures were delivered. At the beginning of the study the clinical condition of the mucosa was recorded, and salivary...
April 2011
Prevalence of plasmid-mediated quinolone resistance qnr genes in Central China
A total of 179 Gram-negative bacteria strains collected in Wuhan from 2005 to 2006 were screened for qnrA, qnrB and qnrS genes by polymerase chain reaction (PCR). The qnrAgenes were detected in 3.88% of Escherichia coli and 7.69% of Enterobacter cloacae, whereas the qnrB genes were found in 6.20% of E. coli and 7.69% of E. cloacae....
April 2011
Influence of dietary thymol and carvacrol preparation and/or an organic acid blend on growth performance, digestive organs and intestinal microbiota of broiler chickens
This experiment was designed to investigate the effects of supplementation with plant extract, either alone or in combination with an organic acid on growth performance, intestinal organ measurements and intestinal microbiology. One-day-old male Ross 308 strain broiler chicken (n=96) were allocated to 4 dietary treatments in a randomized complete block design. Dietary treatments were: (i) basal diet (as a...
April 2011
Metabolic control of lignocellulose degradation by lignocellulose-degradation microbial community MC1
This study was to reveal the limiting factors of lignocellulose degradation and further improve the potential degradation of microbial community MC1, by pH manual adjustment (pHMA) and culture medium partial replacement (CMPR) during the process of rice straw degradation. The results indicated degradation rate in control (5% rice straw) increased, ranging from 33.3 to 67.5% via pHMA method, and for treatment...
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