African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Feasibility of using dot blot hybridization to detect Salmonella InvA, SpiC and SipC directly from clinical specimens

Hang’ombe Bernard Mudenda1*, Ulaya William1, Mwansa James C. L.2, Mubita Charles1, Isogai Nayuta3, Mulenga Evans1, Moonga Ladslav1, Isogai Hiroshi4 and Isogai Emiko5
  1School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia. 2University Teaching Hospital, Department of Pathology and Microbiology, Lusaka, Zambia. 3Department of Agro-bioscience, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan. 4Animal Research Center, Sapporo Medical University, 060-8556, South 1 West 17, Chuoh-ku, Sapporo, Japan. 5Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiya-Machi Aoba-ku, Sendai 981-8555, Japan.
Email: [email protected]

  •  Accepted: 28 March 2011
  •  Published: 18 April 2011

Abstract

 

Pathogenesis of Salmonella depends upon a large number of factors controlled by an array of genes that synergise into actual virulence. The goal of this study was to detectSalmonella invA, spiC and sipC directly from clinical specimens, using the dot blot hybridization assay. We detected invA, spiC and sipC as a one combination from 4.5% (95% CI: 2.21 to 8.64) human feacal and 35.2% (95% CI: 26.4 to 45.0) poultry samples after enrichment. Furthermore the dot blot method had a higher sensitivity than routine culture, before and after enrichment. These results indicate that dot blot hybridization may be used to directly detect Salmonella invA, spiC and sipC in clinical samples.

 

Key words: Salmonella, dot-blot hybridization, spiC, sipC, invA, clinical-samples.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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