May 2010
Escherichia coli phoA gene inactivation, insertion and restoration based on the λ red system
The red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in Escherichia coli K-12 through homologous recombination using linear PCR products. Here, E. coli BW 25113 mutants were created by changing the localization of genes on the genome using a method which contains both inactivation and reinsertion steps. The phoA gene, the...
May 2010
Isolation, recombinant expression and characterization of the dprA gene product in Streptomyces rimosus NRRL 2455
Dihydrodipicolinate synthase (DHDPS) catalyses the first enzymatic step in the lysine-biosynthetic pathway in bacteria, plants and some fungi. In this study, cloning and submission of 3639 bp from Streptomyces rimosus NRRL 2455 to the EMBL database under the accession code EU617017 were carried out. The entiredprA gene was cloned and heterologously expressed in Escherichia coli BL21 (DE3)...
May 2010
Culture-dependent and culture-independent approaches to study the bacterial and archaeal diversity from Jordanian hot springs
The microbial diversity was investigated at five major hot springs in Jordan (Ashounah, Waggas, Zara, Zarqa Ma'in and Afra springs) using both microbiological culture-based and molecular culture-independent approaches. One hundred and thirty two (132) bacterial isolates were obtained and characterized morphologically. Out of 132 isolates, 125 isolates were gram positive rods, while the other seven...
May 2010
Functional characterization of the endolysin gene encoded by Pseudomonas aeruginosa bacteriophage PaP1
An endolysin gene was identified in PaP1, a novel virulent bacteriophage ofPseudomonas aeruginosa. The PaP1 endolysin gene encodes a 186 amino acid protein, containing a peptidoglycan binding domain at its C-terminus. Bioinformatic analysis revealed that PaP1 Endolysin possesses high sequence similarity with many bacteriophage endolysins or endolysin like genes at peptide level....
May 2010
Early detection of ovarian carcinoma by proteome profiling based on magnetic bead separation and matrix-assisted laser desorption/ionization time of flight mass spectrometry
In this study, affinity purification with magnetic beads and matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was used to screen candidate biomarkers of ovarian carcinoma. Mass spectrometry compiled from serum of 30 cases of ovarian carcinomas was compared with that from serum of 10 cases of benign group and13 cases of normal group. Interesting peaks were further sieved...
May 2010
Molecular identification and codon optimization analysis of major virulence encoding genes of Aeromonas hydrophila
Aeromonas hydrophila is an opportunistic pathogen of aquatic and terrestrial animals and human beings. The aim of this study is to investigate 25 isolates of A. hydrophilafrom 40 fish muscles and 12 water samples. All isolates are screened for the presence of virulence factors such as aerolysin, hemolysin and lipase both phenotypically as well as genotypically. All the isolates produced lipase,...
May 2010
Rationalizing some medicinal plants used in treatment of skin diseases
The hydroethanolic extracts obtained from ten plant species being used as components of skin disease remedy in Southwest Nigeria were subjected to phytochemical analysis and screened for antimicrobial activity. The antimicrobial activity was determined against Escherichia coli NCIB 86, Staphylococcus aureusNCIB 8588, Klebsiella pneumoniae NCIB 418, Pseudomonas...
May 2010
Influences of light and temperature on membrane potential and respiratory viability of an aerobic anoxygenic phototrophic bacterium Erythrobacter sp. JL475
Flow cytometry’s (FCM) measurement of membrane potential (MP) and cell respiration viability based on continuous culture was used to investigate the responses of aerobic anoxygenic phototrophic bacteria (AAPB) in the heterotrophic growth and regulation mechanism of photosynthesis to environmental changes. An AAPB strain Erythrobacter sp. JL475 and a non-AAPB...
May 2010
Effects of environmental and nutritional factors on gelatinolytic activity by Enterococcus faecalis strains isolated from clinical sources
Gelatinase is a potential virulence factor in Enterococci which are opportunistic pathogens that cause various types of infections in humans. The production of gelatinase was studied in 95 Enterococcus faecalis strains isolated from different types of clinical sources. Various environmental parameters including culture medium, temperature, pH, divalent cations and carbon sources were examined for...
May 2010
Research advance and superiority of microdiesel production with biowastes
Lignocellulosic biowastes can be utilized to produce microdiesel, a promising alternative energy source for limited crude oil and food based biofuels. As an alternative fuel, microdiesel produced from biowastes it has superior environmental benefits, economically competitive, producible in sufficient quantities and the energy conversion efficiency is also estimated in the article in theory. Microdiesel derived from...
May 2010
Bacteria population of some commercially prepared yoghurt sold in Enugu State, Eastern Nigeria
The demand for fermented dairies is on the increase in developing countries following consumer awareness of some of the health benefits attributed to prolonged intake. Eight samples of yoghurt produced from Enugu, Eastern Nigeria was collected from various locations. Samples were incubated using various media for the isolation, identification and enumeration of the bacteria population within the yoghurt....
May 2010
Monogenean parasites in Sparid fish (Pagellus genus) in eastern Algeria coastline
A spatial distribution study of parasite populations of gills from 540 specimens belonging to Pagellus genus: Pagellus acarne, Pagellus erythrinus and Pagellus bogaraveo (Sparidae) was realized from the eastern Algeria coastline. A preliminary description allows the distinguishing of eight parasite species belonging to two subclasses:Monopisthocotylea and...
May 2010
Characterisation of non human origin probiotic Lactobacillus plantarum with cholesterol-lowering property
This study evaluated the probiotic and cholesterol-lowering property of Lactobacillusspp. isolated from non human origin. Four strains, TGCM 15, TGCM 26, TGCM 33 and TGCM 128 were selected and identified as Lactobacillus plantarum. These strains tolerated to 0.15% and 0.30% (w/v) bile salt and resisted to pH values 2 - 8 with survival rate more than 50% during 2 h of growth. In...
May 2010
Isolating fungal endophyte from Paris polyphylla Smith var. yunnanensis and identifying their antibacterial ability
Eighteen fungal endophytes strains parasitizing the famous Chinese medicinal plantParis polyphylla Smith var. yunnanensis (Franch.) Hand. Mazz were isolated from surface-sterilized plant tissues, such as rhizome, root, stem, leaf and flower. The effect of endophytes on the growth of human pathogenic microbes was evaluated in vitro, using disc diffusion assay....
May 2010
Susceptibility pattern of pseudomonas aeruginosa against various antibiotics
To determine current trends of antibiotic resistance among clinically significantpseudomonas aeruginosa strains causing various nosocomial and community acquired infections. A total of 100 clinical isolates of P. aeruginosa from inpatient and outpatient were studied from June 2008 to May 2009 for its prevalence and susceptibility profiles. Most of the isolates were from pus followed by urine,...
May 2010
Evaluation of serum copper level during Giardia intestinalis infection
Alterations of serum copper concentrations are commonly found in patients suffering from gastrointestinal infections and with hepatic, renal, cardiovascular and malignant diseases. In this study, we aimed to investigate the changes of total content of the essential element of copper level in patients infected with Giardia intestinalis. Copper concentration was measured in 64 patients who were positive...
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