African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5165

Table of Content: 18 October; 4(20)

October 2010

Assessment of the temporal change in groundwater quality when stored at different temperatures in household conditions, in the equatorial region of Central Africa

The study carried out aimed at assessing the impact of groundwater storage temperature at household conditions on the temporal evolution of electrical conductivity and the future of heterotrophic aerobe bacteria (HAB). The storage duration was 7 days and the considered temperatures were 3, 10, 18 and 25°C. Theelectrical conductivity during storage reached 829 µS/cm at 3 - 18°C and 850 µS/cm at...

Author(s):   Moïse Nola, Ernest Djarmaila, Norbert Kemka, Serge H. Zébazé Togouet, Nour-Eddine Chihib, François Krier, Pierre Servais, Jean-Pierre Hornez and Thomas Njiné

October 2010

Bacillus pumilus, a new pathogen on potato tubers in storage in Mali

  Soft rot occurred severely in potato tubers stored in traditional and ameliored storehouses at Sikasso, Mali. 17 infective bacterial isolates were isolated from potato rot tissues (Solanum tuberosum L var. Odessa). Out of all, the isolate Od23 was found pathogenic and was characterized as rod-shaped, Gram positive, endo-spore formers and yellow pigment producers. This isolate which was found to be the...

Author(s): Hamed Bathily, Amadou Hamadoun Babana and Fassé Samaké

October 2010

Dynamics of acid phosphatase production of the ectomycorrhizal mushroom Cantharellus tropicalis

  Ectomycorrhizal mushroom Cantharellus tropicalis was grown in axenic culture to study the effect of pH, temperature, nitrogen, carbon, phosphorus and heavy metals/trace elements on production of acid phosphatase and mycelial growth. The results of present study showed optimum mycelial growth with pH 4 at 15 and 35 ± 2°C. The ectomycorrhizal mushroom mycelia utilized lactose and yeast...

Author(s): Rohit Sharma, Rakesh K. Baghel and Akhilesh K. Pandey

October 2010

Analysis of compatibility relationships among some almond genotypes using fruit set and fluorescence microscopy

  Most of the Iranian almond cultivars are self-incompatible followed by pollination, fertilization, fruit set and lower yield problems. Therefore, selecting suitable cross-compatible cultivars for orchard establishment is necessary especially by new cultivars/genotypes obtained from breeding programs. In this study fruit set and pollen tube growth of ten late-bloom almond genotypes, obtained from a breeding...

Author(s):   Yavar Sharafi, Jafar Hajilou, Seyyed Abol-Gasem Mohammadi and Mohammad Reza Dadpour  

October 2010

Survival enhancement of probiotic Lactobacillus plantarum CMU-FP002 by granulation and encapsulation techniques

  Two processes of enclosure of probiotic Lactobacillus plantarum CMU-FP002, probiotic granules and calcium alginate beads, were studied.  Sodium alginate solution at 1.0, 1.5 and 2.0 % (w/v) was used as a binder.  The results showed that 20 log cfu/ml initial concentration of cells could be entrapped by the granules and beads with 12 to 13 log cfu/g and 16 cfu/g, respectively. The physical...

Author(s):   Sasimar Woraharn, Chaiyavat Chaiyasut, Busabun Sirithunyalug and Jakkapan Sirithunyalug  

October 2010

Does dermatophagoides cause different allergic rhinitis clinic than pollens?

  The aim of this study was to compare allergic rhinitis clinics caused by two most common allergens: house dust mites (HDM) and pollens. Three hundred and fifty patients were evaluated. These patients  were pure pollen or HDM allergic according to skin prick test (51 HDM+, 299 Pollen+). Mainly, HDM were allergens for perennial allergic rhinitis, while pollens were for seasonal allergic rhinitis...

Author(s): M. Akarcay, M. C. Miman, O. Miman, M. Kelles and N. Ekici  

October 2010

Bio-modeling of active site in oxidized azurin using by computational methods

  A cluster model for active site of oxidized azurin was presented and investigated the geometric structure and thermochemical parameters. Quantum-mechanical calculations were performed at the HF and B3lYP/6-31G levels of theory in the gas phase and eight solvents at four temperatures. Also, nuclear shielding parameters of the active site of oxidized azurin have been taken into account using GIAO and CSGT...

Author(s): F. Mollaamin, K. Shahani poor, T. Nejadsattari and M. Monajjemi,

October 2010

Campylobacteriosis in sheep in farm settlements in the Vhembe District of South Africa

  A total of 300 freshly voided sheep faeces were collected and screened for the presence of Campylobacter spp. using standard microbiological techniques. The samples were obtained randomly from 3 farm settlements in the Venda Region, South Africa in 2008 and 2009. The recovery rate was 30.0% (90 of 300) for all faeces. Of these, 65 (72.2%) were from diarrheic and 25 (27.8%) were from...

Author(s):   Uaboi-Egbenni, P. O., Bessong, P. O., Samie, A. and Obi, C. L.  

October 2010

Serological and bacteriological study of leptospirosis in slaughtered cattle in North of Iran (Rasht)

  A serological and bacteriological study was performed in Guilan industrial slaughter house, in Rasht, North of Iran in 2009. To investigate the seroprevalence of leptospirosis in slaughtered cattle in Guilan, 59 and 39 random serum samples were collected from cows and bulls, respectively. None of the cattles was vaccinated against leptospirosis. Also urine samples were collected from all of the blood-sampled...

Author(s): T. Shafighi, G. Abdollahpour, T. Zahraei Salehi and H. Tadjbakhsh

October 2010

Textile effluent treatment by Bacillus species isolated from processed food

Colour removal of industrial effluent has been a major concern in waste water treatment, especially for the waste water that originates from textile and dye stuff plant with a continuous discharge of great quantity of remaining dyes to the environment. The efficient treatment of the effluent is an eco- friendly method for the treatment of textile effluent. Bacillus species was isolated from the processed...

Author(s): N. Murugalatha, A. Mohankumar,  A. Sankaravadivoo  and  C. Rajesh

October 2010

Identification of Streptococcus salivarius bacteriophage isolated from Persian Gulf as a potential agent for dental caries phage therapy

  The aim of this research was to detect oral Streprtococci bacteriophages from Persian Gulf. Dental plaque samples were collected using sterile explorer and cultured in brain heart infusion (BHI) Broth. The oral Streptococci were isolated in culture media. The Persian Gulf water sample was gathered using a sterile bottle from the depth of 50 cm under the inframarine surface at Boushehr...

Author(s): Keivan Beheshti Maal, Majid Bouzari and Farahnaz Arbabzadeh Zavareh

October 2010

In vitro antimicrobial activity of leaves of Acalypha indica Linn (Euphorbiaceae)

  The antimicrobial activity of water, ethanol and chloroform extracts of Acalypha indicawas tested against four bacterial and fungal strains using the disc diffusion method.The antibacterial activity against gram positive bacteria was more pronounced (p < 0.05) in water and ethanol extracts. Antifungal activity was more significant (p < 0.05) only in chloroform extract. This antimicrobial activity...

Author(s):   M. N. Somchit, R. Abdul Rashid, A. Abdullah, A. Zuraini, Z. A. Zakaria, M. R. Sulaiman, A. K. Arifah and A. R. Mutalib  

October 2010

Molecular and biochemical characterization of surfactin producing Bacillus species antagonistic to Colletotrichum falcatum Went causing sugarcane red rot

  Bacillus species suppress phytopathogens by producing lipopeptide antibiotics, hydrolytic enzymes, siderophores and other secondary metabolites. Three bacterial strains Bacillus subtilis NH-100 (EU627167), B. subtilis NH-160 (EU627169) andBacillus sp. NH-217 (EU627170) with proven ability to suppress red rot disease on sugarcane plants were further characterized to elucidate the...

Author(s):   Muhammad Nadeem Hassan, A. Mark Osborn and Fauzia Yusuf Hafeez,  

October 2010

The role of hp1165 gene on the efflux-mediated resistance to tetracycline in clinical isolates of Helicobacter pylori

  Tetracycline-resistant (TetR) Helicobacter pylori isolates have emerged in many parts of the world. We have previously demonstrated that among the mechanisms involved in the resistance of H. Pylori to Tet, its active efflux may be an important mechanism. This work, aimed to determine whether presence and/or expression ofhp1165 are associated with efflux-mediated resistance to Tet in...

Author(s): T. Falsafi, M. Anoushiravani and V. Niknam

October 2010

AdeABC efflux pump: Less important role in Acinetobacter baumannii against carbapenems

  AdeABC efflux pump in a total of 50 Acinetobacter baumannii strains were investigated and the role in contributing to hydrolysis carbapenems were further analyzed. All strains were divided into 3 groups according to their susceptibilities to Imipenem, Amikacin, Minocycline and Levofloxacin: Group A (22 imipenem-resistant strains), B (13 isolates which were imipenem-sensitive but resistant to at...

Author(s): Jiansheng Huang, Jinwei Huang, Fangyou Yu, Xiaoguang Wang and Guoxiong Li

October 2010

Infections and aerobic bacterial pathogens in diabetic foot

  The aim of the study was to investigate the causative pathogens, profile of antimicrobial susceptibility of them and the extent of tissue lesions in diabetic foot infections. This is a prospective study in which infected diabetic foot presenting with Wagner grade 1 to 5 ulcers were investigated. 78 consecutive diabetic patients who were seen in the orthopaedic clinic were cultured during ordinary visits....

Author(s): Ozer B., Kalaci A., Semerci E., Duran N., Davul S. and Yanat A. N.

October 2010

Effect of 60Co γ radiation on mesenchymal stem cells (MSCs) proliferation and differentiation

  Mesenchymal stem cells (MSCs) are undifferentiated multipotent cells which reside in various human tissues and have the potential to differentiate into osteoblasts, chondrocytes, adipocytes, fibroblasts and other tissues of mesenchymal origin. In this study, we investigated the effect of 60Co γ radiation on the proliferation and differentiation of MSCs. MSCs were treated with increasing radiation doses...

Author(s): Xiaohui Ma, Wei Guo, Xiaoping Liu, Tai Yin, Xin Jia, Jiang Xiong, Hongpeng Zhang and Lijun Wang

October 2010

Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis

  Staphylococcus aureus is one of the major pathogens which cause Bovine Mastitis (BM). Probiotic lactobacilli have the great potential to produce antimicrobial compounds that inhibit and control pathogenic bacteria. Antagonistic activity of probiotic lactobacilli (L. acidophilus DSM 20079, L. plantarum ATCC 8014, L. caseiATCC 39392 and L. reuteri ATCC 23272)...

Author(s): Nazila Arbab Soleimani, Rooha Kasra Kermanshahi, Bagher Yakhchali and Taher Nejad Sattari

October 2010

Antimicrobial effect of slightly acidic electrolyzed water for inactivation of Salmonella spp. and Escherichia coli on fresh strawberries (Fragaria L.)

  Antimicrobial effect of slightly acidic electrolyzed water (SAEW: pH 5.6 ± 0.1, 20.5 ±1.3 mg/L available chlorine concentration; ACC) against indigenous aerobic mesophiles and inoculated Escherichia coli and Salmonella spp. on fresh strawberry was assessed. The antimicrobial effect of SAEW was compared with that of strong acidic electrolyzed water (StAEW) and...

Author(s):   Abdulsudi Issa-Zacharia, Yoshinori Kamitani, Happiness Muhimbula andKoichi Iwasaki  

October 2010

Inhibition of Astragalus membranaceus polysaccharides against liver cancer cell HepG2

  We have studied the inhibition of polysaccharides of Astragalus membranaceusagainst liver cancer cell HepG2. The polysaccharides at higher doses (25 mg/ml) have stronger antitumour effects, decreasing more than 40.5% (24 h) and 67.3% (48 h) of liver cancer HepG2 cell viability. A high percentage of apoptotic HepG2 cells was found at 25 mg/ml of A. membranaceus polysaccharides. 23.9 and 38.2% of...

Author(s): Wu Yejin and Wang Yanqun

October 2010

Identification and characterization of tenuazonic acid as the causative agent of Alternaria alternata toxicity towards groundnut

  Alternaria alternata (Fr.) Keissler, the causal agent of Alternariosis of groundnut seeds (Arachis hypogaea L.) was toxigenic when cultured on various laboratory media. The cell free extract or culture filtrate induced severe chlorosis and necrosis on leaves, inhibition of root and shoot growth of germinating seeds and wilting of seedlings. A phytotoxin was isolated both from cell free...

Author(s):   P. Suvarnalatha Devi, M. N. Reddy, M. Nagalakshmi Devamma, P. Jayalakshmi, G. Revathi and G. Narasimha