African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5123

Full Length Research Paper

Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis

Nazila Arbab Soleimani1*, Rooha Kasra Kermanshahi2, Bagher Yakhchali3 and Taher Nejad Sattari1
  1Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of Microbiology, Alzahra University, Tehran, Iran. 3National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.  
Email: [email protected]

  •  Accepted: 17 September 2010
  •  Published: 18 October 2010

Abstract

 

Staphylococcus aureus is one of the major pathogens which cause Bovine Mastitis (BM). Probiotic lactobacilli have the great potential to produce antimicrobial compounds that inhibit and control pathogenic bacteria. Antagonistic activity of probiotic lactobacilli (L. acidophilus DSM 20079, L. plantarum ATCC 8014, L. caseiATCC 39392 and L. reuteri ATCC 23272) against S. aureus isolated from bovine mastitis (BM S. aureus) and standard S. aureus ATCC 25923 was the objective of this study. Antagonistic effect of probiotic lactobacilli was investigated by modified double layer method, well diffusion method, co-culturing assay and co-aggregation method. Among four lactobacilli, L. plantarum showed the greatest inhibitory activity. In modified double layer method the zone of inhibition of BM S. aureus and standardS. aureus ATCC 25923 by L. plantarum was 44 and 40 mm, respectively. Cell Free Supernatant (CFS) of probiotic lactobacilli in well diffusion method had inhibitory effect. Inhibition zone of BM S. aureus (13 mm) and standard S. aureus ATCC 25923 (9 mm) by L. plantarum was achieved. Co-culturing of L. plantarum with these two bacteria resulted in 87 and 77% inhibition growth of BM S. aureus and standard S. ureus ATCC 25923, respectively after 12 h. Co-aggregation between L. plantarumwith two mentioned S. aureus was obtained 88.4 and 76%, respectively. According to these data, L. plantarum and its antimicrobial compounds can be one of the selective choices to control the BM S. aureus.

 

Key words: Antagonistic effect, probiotic lactobacilli, bovine mastitis, Staphylococcus aureus.