African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5034

Table of Content: 19 July, 2012; 6(27)

July 2012

Evaluation of biodegradation of 2-chlorobenzoic acid by isolated bacteria from landfill soils in Shiraz, Iran

  Chlorobenzoic acids are one of the environmental pollutants which contaminate the soils and waters. Microorganisms have been used for removing pollution in the area of oil spill, nuclear waste, leakage from waste landfill, factory waste, and heavy metals. The aim of this study was to identify 2-chlorobenzoic acid degrading bacteria from the soils around the landfill centers (landfills) in Shiraz city (Iran)...

Author(s): Farshid Kafilzadeh, Mahnaz Nikvarz, Sepideh Jabbari and Yaghoob Tahery

July 2012

Genetic analysis and comparative virulence of isolates of Pyrenophora tritici-repentis from wheat in Argentina

  Tan spot caused by the fungus Pyrenophora tritici-repentis, occurs in many wheat-growing regions worldwide and is considered among the major diseases of wheat in Argentina. In nature, this pathogen reproduces both sexually and asexually, but the extent of genetic variability in the P. tritici-repentis Argentinian population is unknown. The genetic structure of P....

Author(s): A. Perelló, M. Yanniccari, MV. Moreno, and AM. Castro

October 2013

Arbuscular mycorrhizal fungi: Essential belowground organisms for earth life but sensitive to a changing environment

  Changes in the soil and environment rarely occur in isolation and new insights into their impacts on arbuscular mycorrhizal fungi (AMF) in moderating ecosystems processes are needed. Any climate, soil and/or plant type change affects activity and biomass of extra radical mycelia of AMF and can consequently impact on the physiology of the host plant as well as on soil structure and rhizospheric microorganisms....

Author(s): Covacevich Fernanda, Echeverría Hernán E.  and Pagano Marcela C.  

July 2012

Production of indole acetic acid by Rhizobium isolates from Vigna trilobata (L) Verdc.

  Rhizobium strains were isolated from root nodules of five cultivars of Vigna trilobatanamely: cultivar-1 from Prakasham District, cultivar-2 from East Godavari District, cultivar-3 from Guntur District, cultivar-4 from West Godavari District and cultivar-5 from Krishna District on yeast extract mannitol broth (YEMB) medium with bromothymol blue indicator. The strains were examined for production of...

Author(s): P. Ravi Kumar and M. Raghu Ram

July 2012

Production and characterisation of protease enzyme produced by a novel moderate thermophilic bacterium (EP1001) isolated from an alkaline hot spring, Zimbabwe

  A moderately thermophilic bacterium designated EP1001 isolated from an alkaline hot spring (conditions in the hot spring: temperature 53-54°C and pH 9.3) in Zimbabwe was investigated for protease production. Studies to determine the growth and protease enzyme production by the novel bacterium were carried out in ordinary batch, regulated batch and fed-batch modes of fermentation using a BioFlow III 3 L...

Author(s): Parawira Wilson, and Zvauya Remigio

July 2012

Molecular characterization of nosocomial CTX-M type ß–Lactamase producing Enterobacteriaceae from University Hospital in Turkey

  Extended-spectrum ß–Lactamase (ESBL) producing members of the Enterobacteriaceae family are important nosocomial pathogens. CTX-M type extended-spectrum ß–Lactamases (ESBLs) are now the most important cause of resistance to beta-lactam antibiotics in Escherichia coli and are reported worldwide with increasing frequency. Dominant CTX-M genotype varies geographically. CTX-M 15...

Author(s): Cihangir Gulamber, Mustafa Altindis, Raike Kalayci, Bulent Bozdogan and Orhan Cem Aktepe

July 2012

In vitro bacteriologic study and empiric antibiotic regimens for diabetic foot ulcers

  Hundred diabetic patients were admitted with clinically infected foot ulcers and were studied during the period of 1st January 2010 to 30th June 2011. Pus samples of bacterial culture were collected from 30 patients admitted with diabetic foot infection. Antimicrobial susceptibility testing of aerobic isolates was performed by the standard disc diffusion method as recommended by National Committee...

Author(s): Ishrat Bano, Waqas Ashraf Chaudhary and Abdul Hameed

July 2012

Study on the combined insecticidal effect of pyrethroid, Azadirachta indica and boric acid on the Bacillus thuringiensis efficacy in Tribolium castaneum

  The red flour beetle (Tribolium castaneum) is a common pest insect known for attacking and infesting stored flour and grain. Bacillus thuringiensis (B.t) is a Gram positive, soil dwelling bacteria commonly used as a biological alternative to a pesticide. The present project was designed for the study of combined insecticidal effect of B. t along with boric acid, insecticide...

Author(s): Kausar Malik, Sidra Nazir, Amjad Farooq, Farkhanda Jabeen, Shagufta Andleeb and Mir Muhammad Ali Talpur

July 2012

Rapeseed meal feeding effects on total proteins and lipids of Japanese Quail

  A detailed study was undertaken, in order to determine the safe dose of mustard seed meal required in the diet of Japanese quails that can be used at the production level without having deleterious effects on the growth of these birds. For this purpose, some 1500 birds were fed different levels of mustard seed meal in isocaloric and isonitrogenous diets. The results of its application, on the diets of these...

Author(s): Kausar Malik, Khalid Pervaiz Lone, Amjad Farooq, Asmat Ullah, Shagufta Andleeb,Muhammad Ali Talpur, Naeem Rashid, Khansa Nakhshab Choudhary and Khadija Awan

July 2012

Toxicological effects of silver nanoparticles in rats

  Toxicity of nanoparticles depends on chemical composition, atomic arrangement and particle size. Silver is being widely used in consumer medical products, due to its uniqueness such as antimicrobial activity. In this study, we assessed the toxic effects of size-silver nanoparticles 70 nanometer in rat’s living tissues, with different doses such as 0.25, 0.5, 1 and 2 mg/kg body weight...

Author(s): Roshan Rezaee Ranjbar Sardari, Saeid Rezaei Zarchi, Alireza Talebi, Sima Nasri, Saber Imani, Arezoo Khoradmehr and Seyed Alireza Razavi Sheshde

July 2012

Prevalence study of Coxiella burnetii in aborted fetuses of small ruminants in various partum and seasons in Iran

  Query (Q) fever is an ubiquitous zoonosis caused by Coxiella burnetii, an obligate intracellular rickettsial organism that cause abortion and stillbirth in ruminants. The prevalence of C. burnetii in Iran is essentially unknown. Its traditional diagnosis is based on culture, serology and conventional polymerase chain reaction (PCR). In this present study, for more sensitive and accurate...

Author(s): Farhad Safarpoor Dehkordi and Mojtaba Salari Rafsanjani

July 2012

Clinical microbiology study of diabetic foot ulcer in Iran; pathogens and antibacterial susceptibility

  The aim of this study was to investigate microbial pathogens and their antibiotic susceptibility profile in infected diabetic foot ulcers in Iranian patients. This was a one-year cross sectional study on diabetic patients with infected diabetic foot ulcer at Shariati Teaching Hospital, Tehran, Iran. Grade of ulcer was determined by Wagner’s criteria. Specimens were obtained from the base of ulcer, deep...

Author(s): Nahid Rouhipour, Alireza Hayatshahi, Mohsen Khoshniat Nikoo, Nika Mojahed Yazdi, Ramin Heshmat, Mostafa Qorbani, Masoud Mehrannia, Abolfazl Shojaifard, Farzaneh Abbasi, Seyed Mohammad Tavangar, Mohammad Reza Mohajeri Tehraniand Bagher Larijani

July 2012

Evaluation of different deoxyribonucleic acid (DNA) extraction methods using dried blood spot for early infant diagnosis of HIV1 in Sub-Saharan Africa

  Mother-to-child transmission leads to death of 50% of infected children in the first 2 years of life. Early diagnosis is essential for timely treatment. Dried blood spots (DBS) collected on filter paper provide a simple approach for sample collection especially in resource limited settings, because storage and shipment are very easy. The aim of the study was to standardize reproducible,...

Author(s): Ceffa Susanna, Erba Fulvio, Brambilla Davide, Luhanga Richard, Armel Pépé Mamy, Paturzo Giovanna, Nielsen-Saines Karin, Mancinelli Sandro, Narciso Pasquale, Palombi Leonardo and Marazzi Maria Cristina

July 2012

Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in Hawassa area, South Ethiopia

  Quarter milk samples from cows were examined to determine the prevalence ofStaphylococcus aureus (SA) and different antibiotic resistant pattern were determined in a cross-sectional study design. The aim of this study was to isolate Staphylococcus aureusfrom samples of cow’s milk obtained from Hawassa area and to determine their antibiotic susceptibility patterns. A total of 160 milk...

Author(s): Deresse Daka, Solomon G. Silassie and Dawit Yihdego

July 2012

Oxidative stress and altered levels of antioxidants in adolescents with Down syndrome during pre-exercise and post-exercise

  The aim of this study was to determine the influence of exercise on activity of erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and plasma malondialdehyde (MDA), β-carotene, retinol levels in adolescents with Down syndrome (DS). Twenty adolescents with DS (14.86 ± 7.07 years) performed a 12-week training program. Body composition was measured by bioelectric...

Author(s): Ulku Ozbey, Cengiz Arslan, Yüksel Savucu, Mine Erisir and Ayşe Seyran

July 2012

The relationship between IL-17 and male infertility: Semen analysis

  The relationship between IL-17 and male infertility was investigated. Seminal fluid from 57 males, including infertile and normal, were selected and subjected to routine analysis and determination of the levels of cytokines. IL-17 can be detected in seminal fluid, with concentrations from 0.45 to 16.67 pg/ml. Semen analysis showed that semen samples with low activity sperms have higher IL-17 level, while...

Author(s): Li Qian, Qingxi Shi, Yang Gu, Jicheng Song, Minglian Zhou and Maofang Hua

July 2012

Efflux inhibitory activity of flavonoids from Chromolaena odorata against selected methicillin-resistant Staphylococcus aureus (MRSA) isolates

  Ten flavonoid compounds isolated from the methanolic extract of a tropical weed plant,Chromolaena odorata leaves were tested against three multidrug-resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) isolates with active efflux properties. Only low inhibitory potential were detected through the preliminary evaluation as indicated by their respective minimum inhibitory...

Author(s): Saiful Azmi Johari, Ling Sui Kiong, Mastura Mohtar, Mazurah Mohamed Isa, Salbiah Man, Shuhaimi Mustafa and Abdul Manaf Ali

July 2012

Genetic variation of Fusarium oxysporum f. sp. lycopersici isolated from tomatoes in Thailand using pathogenicity and AFLP markers

  Genetic variation among 25 isolates of Fusarium oxysporum f. sp. lycopersici causing tomato wilt was determined using pathogenicity test and Amplified Fragment Length Polymorphisms (AFLP) markers. The isolates were collected from 8 provinces in Thailand. Based on the pathogenecity result, all isolates were divided into two groups as pathogenic and non-pathogenic isolates. Cluster...

Author(s): Phoutthasone Sibounnavong, Jintana Unartngam and Kasem Soytong

July 2012

Azadirachta indica extracts influenced some pathogenic fungi

  Active components of neem leaves and seeds were extracted with different methods in order to study the effect of different extract concentrations on the inhibition of some pathogenic fungi. High-performance liquid chromatography (HPLC) was used to identify the active components of neem extract. Highest inhibition percentage of ethanolic neem leaf extract was recorded with Rhizoctonia solani, while the...

Author(s): Emad M. El-Kholie, Mohammed A. T. Abdelreheem and Seham A. Khader

July 2012

Pathogenicity of Metarhizium anisopliae towards Rhipicephalus (Boophilus) microplus under laboratory and field conditions

  Metarhizium anisopliae is one of the most studied agents of biological control of several arthropod plagues, including the cattle tick Rhipicephalus (Boophilus) microplus. Studies have been conducted to assess the fungal complex infection process towards its hosts. To accomplish that, mutant strains overexpressing or lacking assumed determinant genes for the process were constructed over...

Author(s): Juliano Tomazzoni Boldo, Roberta Alvares Campos, Lúcia Rosane Bertholdo Vargas, João Lúcio Azevedo, and Neiva Monteiro de Barros

July 2012

Antioxidant effect of celery against carbontetrachloride induced hepatic damage in rats

  Ethanolic extract of celery (Apium graveolens) and its diet were evaluated for antioxidant and hepatoprotective activities in rats. Celery is valuable in weight loss diets and regulate lipid metabolism. Albino male rats were used to evaluate its antioxidant and hepatoprotective activities against carbon tetrachloride induced toxicity. Chromosomal aberration, sperm abnormalities, biochemical and molecular...

Author(s): Abdou H. S., Salah S. H., Hoda Booles F. and Abdel Rahim E. A.

July 2012

First report of Mycoplasma bovis infection in dairy cattle in Guangzhou, subtropical southern China

  Mycoplasma bovis is a major bacterial pathogen causing mastitis in dairy cattle, pneumonia and arthritis, and reduced weight gain in calves and reproductive problems in both dairy cattle and bulls, resulting in significant economic losses. The objective of the present investigation was to examine the M. bovis seroprevalence in dairy cattle in Guangzhou, subtropical Southern China by...

Author(s): Fu-Rong Zhao, Xue-Liang Zhu, Min-Jun Xu, Si-Yang Huang, Dong-Hui Zhou,Hui-Yan Xia, Hui-Qun Song, and Feng-Cai Zou

July 2012

Activities of antioxidants enzymes in Salicylic acid treated tomato against Xanthomonas vesicatoria

  Tomato bacterial spot caused by Xanthomonas vesicatoria is an important disease in many tomato production areas in the world. The potentiality of salicylic acid (SA) to suppressing bacterial spot of tomato (Lycopersicon esculentum L. var.) under artificial inoculation conditions was investigated in the current work. Tomato plants cv. Super Strain B were sprayed with SA (10...

Author(s): Yasser E. Ibrahim

July 2012

Effects of plant growth promoting rhizobacteria on maize in greenhouse and field trial

  The aim of the study was to investigate ability of Pseudomonas sp. Q4b, Bacillus sp. Q5a and Azotobacter chroococcum strain 8 to enrich a population of plant growth promoting rhizobacteria (PGPR) in rhizosphere and thus enhance maize growth (Zea mays L.). Experiments were carried out under greenhouse and field conditions, on a chernozem soil. In...

Author(s): Mirjana Jarak, Nastasija Mrkovački, Dragana Bjelić, Dragana Jošić, Timea Hajnal-Jafari and Dragana Stamenov

July 2012

In vitro evaluation of various fungicides against Fusarium solani isolated from Dalbergia sissoo dieback

  Shisham (Dalbergia sissoo Roxb.) infected with dieback disease is considered as one of the most severe problems in Asia, especially in Pakistan. Some fungi including Fusarium solani are thought to be a causative organism for this epidemiological problem. In our previous studies, we recognized for the first time, F. solani as an etiological agent for theD. sissoo dieback...

Author(s): Nasir Ahmed Rajput, Mumtaz Ali Pathan, Abdul Mubeen Lodhi, Daolong Dou, Tingli Liu, Muhammad Shahid Arain and Faheem Uddin Rajer,

July 2012

Antioxidant, antimicrobial and cytotoxicity studies of Russelia equisetiformis

  In the present study of Russelia equisetiformis belonging to the family scrophulariaceae, leaves were evaluated for antioxidant, antimicrobial and cytotoxicity studies. The leaves were extracted with absolute methanol and further fractionated by solvent-solvent extraction method with increasing polarity based absolute solvents, that is, n-hexane,chloroform, ethyl acetate...

Author(s): Muhammad Riaz, Nasir Rasool, Iftikhar Hussain Bukhari, Muhammad Shahid, Ameer Fawad Zahoor, Mazhar Amjad Gilani and Muhammad Zubair

July 2012

Evaluation of antiviral effect of atorvastatin on H1N1 infection in MDCK cells

  Influenza virus causes annual epidemics and occasional pandemics despite developing new vaccines and antiviral drugs, thus presents significant public health concerns. For efficient treatment of influenza, researchers have shown interest on drugs with pleiotropic effects. Clinical efficacy of all conventional drugs in this case is ambiguous. Atorvastatin as a common drug used to treat hypercholestrolemia can...

Author(s): Parvaneh Mehrbod, Aini Ideris, Abdul Rahman Omar, and Mohd Hair-Bejo

July 2012

First report of Chlamydophila seroprevalence in house sparrows (Passer domesticus) in Lanzhou, Northwest China

  Chlamydophila is an obligate intracellular bacterium, found in a wide range of birds, which is frequently transmitted from infected birds to humans. In the present study, a total of 313 serum samples from free-living, clinically healthy house sparrows were examined using an indirect hemagglutination assay (IHA). Antibodies against Chlamydophila were found in 33 (10.54%) of 313 serum...

Author(s): Wei Cong, Dong-Hui Zhou, Yi-Ming Tian, Min-Jun Xu, Song-Ming Wu, Hui-Qun Song, Xing-Quan Zhu,, Quan Zhao and Ai-Dong Qian

July 2012

A post harvest disease of Mangifera indica fruit caused by Pestalotiopsis mangiferae, in Saudi Arabia

  Mango (Mangifera indica) is one of the most important import fruit of Saudi Arabia. Brown black rots were found as a post harvest disease on the mango fruit. On the basis of the morphological characters, the fungus causing this disease was identified asPestalotiopsis mangiferae (Henn). The disease was tested with the Kochs postulates. This is the first report of fruit rots caused by P....

Author(s): Humaira Rizwana, Iffat Siddiqui and Najat Bukhary

July 2012

Detection of subgroup J avian leukosis virus gene by polymerase chain reaction from whole blood without DNA extraction

  Avian leukosis virus subgroup J (ALV-J) is an exogenous avian leukosis virus that causes significant economic losses. In this study, a whole blood polymerase chain reaction (PCR) assay without DNA extraction was developed, optimized, and was compared with conventional methods for the detection of ALV-J infection. The evaluation assay indicated that the whole blood PCR had similar sensitivity and specificity...

Author(s): Kun Qian, Bolin Hang, Haiyu Shen, Wenjie Jin and Aijian Qin