November 2013
Bioformulation Pseudomonas fluorescens SP007s against dirty panicle disease of rice
Two-different carrier formulations, kaolin and talc-based products were developed with Pseudomonas fluorescens SP007s biocontrol agent. SP007s viability in different carriers stored at room temperature (28 to 33°C) slowly declined to approximately 46.2 and 61.0% after 12-month-old shelf life. The decreased population was first found in five and month months of storage for kaolin and talc-based formulations,...
November 2013
Nucleotide excision repair and photoreactivation in sugarcane endophyte Gluconacetobacter diazotrophicus strain PAL5
Nucleotide excision repair is a DNA repair mechanism mediated by three proteins encoded by the uvrABC gene homologues. This study shows that the mutant B12 of the diazotrophic sugarcane endophyte Gluconacetobacter diazotrophicus, carrying a transposon insertion in a uvrC homologue is hypersensitive to ultraviolet C irradiation. The exposure of irradiated mutant cells to visible light partially reverts the...
November 2013
Isolation and identification of bacteria from Xylosandrus germanus (Blandford) (Coleoptera: Curculionidae)
Biological control studies have been increasingly performed against agricultural and forest pests. To develop a biological control agent, bacteria was isolated from harmful pests and identified using various tests. Xylosandrus germanus (Blandford, 1894) (Coleoptera: Curculionidae) is a harmful pest in the hazelnut orchards and other fruit-tree cultures. In this study, we identified 16 bacteria isolates from healthy X....
November 2013
The effect of hydroxycinnamic acids on growth and H+-ATPase activity of the wine spoilage yeast, Dekkera bruxellensis
Hydroxycinnamic acids are lipophilic compounds naturally present in grape must, and proposed to have antimicrobial properties. Consequently, microorganisms that grow in media containing these acids must have efficient adaptation mechanisms. In Saccharomyces cerevisiae hydroxycinnamic acids enter into the cell where they are deprotonated causing a decrease in internal pH, this variation in the intracellular pH is...
November 2013
Virulence characteristics of Escherichia coli isolates obtained from commercial one-week-old layer chicks with diarrhea
Ninety (90) strains of Escherichia coli were isolated from intestine with fecal contents and liver of seven days old commercial layers presenting diarrhea, and their pathogenicity was determined by in vivo inoculation into the air sacs of day-old chicks. The test revealed 44 strains with high and intermediate pathogenicity level that were analyzed by PCR for the presence of eight virulence genes, and their serogroups...
November 2013
A novel Alcaligenes faecalis antibacterial-producing strain isolated from a Moroccan tannery waste
Drug resistance of many harmful bacteria still represents a real public health problem. This is why the development of new bioactive substance becomes urgent. To reach our goal, a screening of bioactive substance produced by microorganism, was undertaken. In this work, we reported data on a bacterial strain isolated from the tannery of Fez (Morocco) that showed a broad antagonistic effect against a group of...
November 2013
Phenotypic and genotypic characterization of methicillin and vancomycin resistant staphylococci
One hundred (100) staphylococci were isolated from several Egyptian hospitals and laboratories which include 85 clinical isolates and 15 from hospital surroundings. The isolates were identified by conventional and molecular techniques. Fifty (50) isolates were identified as Staphylococcus aureus (SA), 40 were Staphylococcus epidermidis (SE) while 10 were identified as Staphylococcus species (SS). Upon testing the...
November 2013
Water condition and identification of potential pathogenic bacteria from red tilapia reared in cage-cultured system in two different water bodies in Malaysia
The need to conduct periodic surveillance on the presence, associated pathological alteration in tissues and the various environmental factors that could trigger some potential fish pathogens that result to disease outbreak in tilapia farming in Malaysia is paramount. This study was conducted to determine the presence of potential pathogenic bacteria in fish and water bodies that could trigger disease outbreak. Some...
November 2013
Use of combination of bacteriocins from Lactobacillus plantarum MTCC 1407 and Bacillus coagulans MTCC 492
Bacteriocins are antimicrobials produced mainly by lactic acid bacteria as well other genera, a property which can be exploited in food biopreservation. However, narrow spectrum of activity of these bacteriocins is a limitation for their use in different food systems. Various approaches are being pursued to increase their antimicrobial efficacy like use of increased dosage, use of highly purified form in combination...
November 2013
Endophytic fungi from medicinal herb Salvia miltiorrhiza Bunge and their antimicrobial activity
A total of 57 endophytic fungal isolates were obtained from the roots of Salvia miltiorrhiza Bunge (Lamiaceae). Fourteen (14) distinct isolates were selected for further taxonomical identification by morphological traits and internal transcribed spacer (ITS) rRNA gene sequence analysis. Twelve (12) genera were identified among which Alternaria and Fusarium were dominants. Eight endophytic fungi (that is, Pleosporales...
November 2013
Detection of icaA and icaD genes and biofilmformation in Staphylococcus spp. isolated from urinary catheters at the University Hospital of Tlemcen (Algeria)
Staphylococcus spp. is a major cause of infections associated with urinary catheterization and other medical devices. Biofilm formation is an important step in the pathogenesis of these Staphylococci and depends on the expression of the icaADBC operon involved in the synthesis of a polysaccharide intercellular adhesion. In this study, forty-four (44) Staphylococcus spp. obtained from urinary catheters at the University...
November 2013
Hirudo verbana is a source of fungal isolates potentially pathogenic to humans
As microbial contamination of leeches poses a risk of transmission of pathogens to humans, contact with non-sterile leeches causes a potential hazard to human health. The purpose of this study was to evaluate the mycological purity of the body surface, jaws/pharynx and intestines of the medical leech, Hirudo verbana, and the purity of aquarium water within which the leeches were incubated. Leeches were kept without...
November 2013
Antifungal activity of secondary metabolites of Pseudomonas fluorescens isolates as a biocontrol agent of chocolate spot disease (Botrytis fabae) of faba bean in Ethiopia
Pseudomonas fluorescens isolates possess a variety of promising properties of antifungal activity of secondary metabolites which make it as a biocontrol agent. In the present study, 12 isolates of P. fluorescens were isolated from rhizospheric soil of faba bean crop evaluated for their antagonistic activity against chocolate spot disease (Botrytis fabae) of faba bean. P. fluorescens 10 (Pf 10) (88.1%) showed high...
November 2013
In vitro degradation of natural animal feed substrates by intracellular phytase producing Shiwalik Himalayan budding yeasts
Himalayas are the natural reservoir of complex and diversified gene pool. Three Shiwalik Himalayan intracellular phytase producing budding yeasts were assayed for in vitro degradation of natural animal feed substrates. Phosphorus availability was found to enhance upto 70% yeast cultures during in vitro biodegradation of natural animal feed substrates. A direct correlation between intracellular phosphate concentration...
Advertisement
Advertisement
