Abstract

Drug resistance of many harmful bacteria still represents a real public health problem. This is why the development of new bioactive substance becomes urgent. To reach our goal, a screening of bioactive substance produced by microorganism, was undertaken. In this work, we reported data on a bacterial strain isolated from the tannery of Fez (Morocco) that showed a broad antagonistic effect against a group of Gram-negative and positive bacteria especially Mycobacterium smegmatis. The antibacterial compounds producer, BW1 was identified as Alcaligenes faecalis on the basis of phenotypic characteristics, biochemical analyses and PCR amplification of 16 S ribosomal RNA gene followed by sequencing (100% of homology). The antibacterial compounds were synthesized during the exponential growth phase of A. faecalis. The antibacterial compounds were not affected following heat treatment and protolytic enzymes that indicated the non-proteinaceous nature of the active agents. The bacterial extract was assayed for cellular toxicity to fresh human erythrocytes and found to have no hemolytic effect.

Key words: Bacterial resistance, antibacterial compounds, Alcaligenes faecalis.