December 2014

Chemical composition and antifungal activity of leaf powders and extracts of Byrsonima crassifolia (L.) Kunth, Ocimum basilicum L., Persea americana Miller., Psidium guajava L. and Spondias purpurea L. from Morelos, México

Natural products from plants have great potential as novel fungicide sources for controlling pathogenic fungi. The aims of this study were to examine the chemical composition of the leave powders and extract (Hexane, methanol and water) of Mexican plants and to test in vitro, their efficacy as antifungal potential against Fusarium oxysporum f. sp. gladioli. All the species showed antifungal activity in the methanol...

Author(s): Claudia Garduño Pizaña, Laura Barrera Necha, Silvia Bautista Baños, Ma. Yolanda Ríos Gómez and Laura García Barrera

December 2014

Eradication of biofilms formed by bacteria isolated from diabetic foot infections by potential antibiofilm agents alone and in combination with ciprofloxacin

This study was performed to investigate the resistance of biofilm forming bacteria isolated from diabetic foot infection to different antibiotics and the eradicating activity of some potential antibiofilm agents alone and in combination with ciprofloxacin. Imipenem was the most active against biofilms formed by all tested strains, while tetracycline was the least active. For biofilms of Gram-positive bacteria,...

Author(s): Hisham A. Abbas and Amany I. Gad

December 2014

Immobilization of dextranase by Aspergillus penicillioides NRC 39 and its properties

Dextranase from Aspergillus penicillioides NRC 39 was immobilized using different carriers by various techniques including physical adsorption, covalent binding, ionic binding and entrapment. Immobilization of dextranase by covalent binding using 2% glutaraldehyde on prawn shell as carrier produced a high immobilization yield 87.4%. Comparison between the free and immobilized enzyme showed that immobilized enzyme on...

Author(s): Aliaa R. El-Shamy and Sherien M. M. Atalla

December 2014

In silico identification of 44 species and subspecies of staphylococci by restriction analysis of the gap gene polymorphism using HpyCH4V enzyme

The aim of this study was to detect and analyze in silico species/subspecies polymorphisms in the sequences of staphylococcal gap gene using HpyCH4V restriction enzyme. We analyzed 64 deposited in the GenBank sequences of the gap gene, derived from 41 staphylococcal species and 23 staphylococcal subspecies. We also attempted to develop and describe specific RFLP profiles of the gap gene for each of the analyzed...

Author(s): Jolanta Karakulska and Karol Fijałkowski