March 2010
Study on the function of a plasmid harbored by a newly isolated Bacillus subtilis
With a view to exploring whether there was a correlation between a novel antibiotic and a plasmid, the plasmid was eliminated by SDS. Because the elimination of the plasmid had no effect on the bacterium’s producing antibiotic, it was determined that the plasmid had nothing to do with the antibiotic. However, with the plasmid being eliminated, the ability of the bacterium to secrete a kind of...
March 2010
Antagonistic activity of a novel antibiotic against Mycobacterium tuberculosis
The antibiotic produced by a newly isolated Bacillus subtilis has potent anti-tubercular activity against sixteen strains of Mycobacterium tuberculosis, most of which belong to MDR strains. MICs for the sixteen strains were evaluated. A series of spectrumanalysis and element analysis determined that the antibiotic’s molecular formula was C27H46O2. Key...
March 2010
Inhibitory effect of aqueous garlic extract (Allium sativum) on some isolated Salmonella serovars
There has been a consistent increase in the search for alternative and efficient compound for food conservation, aiming a partial or total replacement of antimicrobial chemical additives. Garlic offers a promising alternative for food safety and bioconservation. Filter sterilized, aqueous garlic extract was tested for ability to inhibit the growth of some isolated Salmonella serovars. The aqueous...
March 2010
Prevalence of job stress among primary school teachers in South-west, Nigeria
The study determined the job stress among primary school teachers in south west, Nigeria, A total of 624 teachers were chosen as subjects using a multistage sampling technique, and a structure questionnaire was used to gather information from the subjects. Research hypotheses were formulated and inferential statistics of test and Analysis of variance ANOVA were employed to analyze significant differences...
March 2010
Factors affecting synonymous codon usage bias in the gC gene of DPV CHv strain
The aim was to identify the factors affecting synonymous codon usage bias in the newly discovered gC gene of duck plague virus (DPV) CHv strain and a comparative analysis of the codon usage bias in the gC genes of 27 other reference herpesviruses was performed by using CAI, CHIPS and CUSP program of EMBOSS. The results showed that base composition, mutational bias and natural selection were the major...
March 2010
Genomic fingerprints analysis of coagulase-positive and negative Staphylococci isolated from patients with bacteremia by repetitive sequence based PCR method
Staphylococci are important organisms involved in many infections, including bacteremia or septicemia. Repetitive sequence–based PCR (rep-PCR) is a useful method for detection of staphylococcal DNA fingerprint patterns, especially when the origins of these organisms are unknown. Staphylococcal positive blood cultures were collected from patients with bacteremia hospitalized in four hospitals. The...
March 2010
Investigation of a measles outbreak in a rural Nigerian community – The Aladura experience
The global burden of measles has remained a public health challenge. Worldwide, measles is the fifth leading cause of death among under-five children with an estimate of 197,000 deaths in 2007. In Nigeria, measles is an important cause of childhood morbidity and mortality. Measles outbreaks have been increasingly common in the country with 30, 194 and 256 outbreaks reported in 2006, 2007 and 2008...
March 2010
Studies on mycorrhizae development and anatomical confirmation of mycorrhizae formed on Pinus caribaea Mor. var. hondurensis Barr. and Golf. seedlings
The effects of three soil collections from old pine plantations in Nigeria: Enugu, Ugwuoba and Nsukka and a control soil on mycorrhizae development as well as morphology of the mycorrhizal rootlets, anatomical confirmation of mycorrhizae development were investigated. Enugu soil induced the greatest mycorrhizal infection (44%) and growth of seedlings. It is, therefore, recommended for nursery...
March 2010
In vitro antifungal activity of allicin alone and in combination with two medications against six dermatophytic fungi
Dermatophytes are fungi capable of invading keratinized tissues of humans and animals, causing dermatomycosis. Azole antifungal drugs are often used in the treatment of dermatomycosis. Because of increased use of these medications, azoles are known to cause drug resistance; hence this study investigated an alternative anti-dermatophyte which is plant-based, and biodegradable natural product. Allicin is a pure...
March 2010
Current Rhizoctonia solani anastomosis groups in Egypt and their pathogenic relation to cotton seedlings
Twenty eight isolates of Rhizoctonia solani were obtained from cotton seedlings and twenty three isolates from other hosts; eight from peanut, five from chickpea, two from each of flax, tomato and watermelon and one from each of potato, cantaloupe, pepper and lupine. Microscopic examination revealed that 17 isolates (33.33%) each belonged to AG-2-2, 17 and AG-4 HG-I, while 7 isolates (13.73%)...
March 2010
Improved purification and some properties of a novel phospholipase C from Bacillus mycoides strain 970
A novel microbial phospholipase C from Bacillus mycoides strain 970 was purified to apparent homogeneity by an improved purification process, in which DEAE-Cellulose adsorption was first used to remove bulk of dark brown impurities and then a combination of DEAE-Cellulose ion-exchange, Phenyl Sepharose hydrophobic interaction and Sephacryl S-100 gel filtration chromatography was used to separate the...
March 2010
The evolution of cell size distribution during bacterial growth cycle: Experimental observations and individual-based model simulations
Individual-based modelling (IbM) has become a fully incorporated part of predictive microbiology methodologies in the last decade. Previous studies of bacterial culture growth cycle with the IbM simulator INDISIM analysed the evolution of bacterial biomass distribution during the different phases of growth. The predicted forward shift during lag, stability during exponential and backward shift when entering...
March 2010
Mycoflora identified from loggerhead turtle (Caretta caretta) egg shells and nest sand at Fethiye beach, Turkey
The aim of this study was to investigate the mycoflora of the loggerhead turtle,Caretta caretta, from its nests and eggshells at Fethiye beach, Turkey. During the 2004 breeding season, following the complete emergence of the hatchlings, sand samples were collected from 15 nests and eggs from these nests were swabbed. Rose Bengal chloramphenicol agar was used for isolation, and then mycoflora were subcultured...
March 2010
Mycorrhizal root colonisation and the subsequent host plant response of soilless grown tomato plants in the presence and absence of the mycorrhizal stimulant, Mycotech
A hydroponic trial was carried out to assess the effect of inoculating tomato plants with AM fungi, in the presence and absence of a mycorrrhizal stimulant (Mycotech). Four treatments were applied: (1) AM inoculant, (2) mycorrhizal stimulant, (3) AM + stimulant and (4) stimulant alone. Arbuscular mycorrhizal treated plants were 14% root colonized, whilst AM + stimulant treated plants, were 25%...
February 2010
Assessment of synergistic efficacy of carvacrol and cymene against Edwardsiella tarda in vitro and in Tilapia (Oreochromis niloticus)
Carvacrol, a major compound naturally present in oregano and thyme and its precursor, cymene, were tested in vitro for their antimicrobial activity againstEdwardsiella tarda, a fish pathogenic bacteria causing edwardsiellosis. When used alone, carvacrol, but not cymene, inhibited the bacterium with the minimal inhibitory concentration (MIC) of 20 ppm. However, cymene was shown to be able to enhance...
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