March 2014
Different cover promote sandy soil suppressiveness to root rot disease of cassava caused by Fusarium solani
Sandy soils of 19 arable fields were analyzed for disease suppressiveness against root rot disease of cassava, caused by Fusarium solani CFF109. Analysis of chemical, physical, biochemical, microbial population and activity, and soil characteristics were assessed. Soils with different management histories and covers most commonly found in the region were collected to build a greenhouse experiment, to evaluate the...
March 2014
Comparative study on sampling methods for monitoring Legionella species in environmental water
Infections caused by Legionella spp. are considered an emerging public health problem and are linked to high rates of mortality and morbidity, if not properly treated. This study analyzes 76 samples of water in Guangzhou with the aim of obtaining a more efficient means based on culture to isolate the genus Legionella. A comparative assessment of techniques recommended was performed on monitoring Legionella spp. in...
March 2014
Investigation of extracellular antifungal proteinaceous compound produced by Streptomyces sp. 5K10
A Streptomyces sp. strain named 5K10, isolated from soil samples of Kaziranga National Park, Assam, India was found to secrete an antimicrobial proteinaceous compound, showing in vitro antagonistic effect against some pathogenic bacteria, yeasts, dermatophytes and filamentous fungi. Capacity to produce extracellular antimicrobial substances by the strain 5K10 was assessed by conventional spot inoculation method in agar...
March 2014
Impact of media composition and growth condition of antifungal production by Streptomyces ambofaciens S2
Anthracnose caused by fungus Colletotrichum gloeosporioides is an important disease of chilli in Asia countries such as Malaysia. This fungus makes the chilli fruits to be unmarketable. In this study, Streptomyces ambofaciens S2 which gave a high antifungal activity was selected and underwent cultural condition optimization. The optimization involved selecting the best carbon and nitrogen sources, seed age, incubation...
March 2014
Phytochemical screening and antibacterial activity of Solanum paniculatum Linn. against planktonic oral bacteria
This study aimed to determine the phytochemical composition of a root extract of Solanum paniculatum Linn. (SPL), and to evaluate its in vitro antibacterial activity. The extract was characterized by phytochemical screening and thin-layer chromatography. The following oral bacteria were used to determine the minimum inhibitory concentration (MIC) and minimum inhibitory concentration of adherence (MICA) Streptococcus...
March 2014
The cellular components of cucumber seedlings after primed with plant growth promoting rhizobacteria, Bacillus subtilis Bs008
This work aimed at investigating the changes in cellular compositions of cucumber seedlings as enhanced by plant growth promoting rhizobacteria, Bacillus subtilis Bs008 using the Fourier transforminfrared spectroscopy (FT-IR). The objective of this study was to test the hypothesis that Bs008 stimulates production of plant cellular components involved in metabolism and growth development mechanisms. Cucumber vegetative...
March 2014
Antibiotic susceptibility of Escherichia coli and Salmonella strains isolated from raw and curds milk consumed in Ouagadougou and Ziniaré, Burkina Faso
Milk and milk products provide a wealth of nutrition benefits, and are widely consumed worldwide. But raw milks can harbor dangerous microorganisms such as Salmonella or Escherichia coli, that can pose serious health risks to human. In order to assess the prevalence and antibiotics susceptibilities of Salmonella spp. and E. coli isolated from raw milk and curds, a total of one hundred (100) samples were collected in...
March 2014
Cloning and expression of a novel cry gene that is potentially active against nematodes
Biological control may reduce nematode populations to acceptable levels using Bacillus thuringiensis. Thus, the search for new cry proteins that are active on this type of target is highly relevant. To identify novel cry genes, B. thuringiensis isolates were analysed using molecular biology. The isolates with DNA sequences resembling genes that are effective against nematodes were fully sequenced through primer walking....
March 2014
Effect of essential oil of Cinnamomum zeylanicum on some pathogenic bacteria
Several in vitro studies have demonstrated antibacterial activity of essential oils extracted from different medicinal plants. In this work, we performed extraction of essential oil from the bark of Cinnamomum zeylanicum by steam distillation, the identification of their compounds was carried out by gas chromatography coupled with mass spectrometry. The antibacterial effect was evaluated on seven isolates of pathogenic...
March 2014
Isolation, identification and control of osmophilic spoilage yeasts in sweetened condensed milk
In this paper, six yeast strains were isolated from spoilt condensed milk in the agar culture media with high sugar content. By employing API 20C AUX strip, these six isolates were identified as Candida pelliculosa strains. Their growth characteristics were then examined under different culture conditions, including various pH value, temperature, sterilization condition, NaCl and glucose concentrations. Both culture...
March 2014
Morphological, physiological and pathological variations among isolates of Colletotrichum falcatum that cause red rot of sugarcane
Twenty-eight isolates were isolated from samples of different sugarcane varieties infected with red rot during this survey; the culture was maintained on slants and Petri plates at 29±2°C. These isolates showed variability in their optimum radial growth pattern. These isolates were morphologically differentiated into two distinct groups: fifteen had light colour and thirteen had dark colour. Cultural...
March 2014
Specificity of the production of traditional meat products and their safety using polish traditional cured meat products as example
Traditional meat products, particularly cured meat products, represent a specific group of products with large consumers in many countries of the world. The methods of producing them, depending on the country and region from which they originate, make them significantly different from their industrially produced equivalents; they may influence the microbiological quality of such products, making their health safety...
March 2014
The effect of ozone on bacterial vaginosis and how it is affected by ultrastructural changes of cells by transmission electron microscope (TEM)
Three hundred and sixty four (364) high vagina swabs (HVS) were gathered from a hospital in the Central Region of Saudi Arabia. The following samples were identified from the following genera: Escherichia coli, Klebsiella sp., Proteus sp., Lactobacillus sp., Micrococcus sp., Staphylococcus sp., Streptococcus sp. as normal vaginal flora, K. pneumoniae, Streptococcus Group B and S. aureus as the causal agent of bacterial...
March 2014
Lentiviral transgenesis of the leopard gecko, Eublepharis macularius
Lentiviral vectors are an effective method of introducing transgenes into the genome of early stage embryos because they transduce both dividing and non-dividing cells. Lentiviral pseudoparticles containing the coding sequence for the fluorescent protein DsRed were injected into freshly laid leopard gecko eggs. Tissue samples were collected from hatchlings, and the samples were tested for the presence of the transgene....
March 2014
Antibacterial effects of pure metals on clinically important bacteria growing in planktonic cultures and biofilms
Antibiotic resistance in pathogenic bacteria is emerging as an issue of serious concern in bio-medical research as well as food and health organizations. Metal complexes are currently being employed in medical devices for their inhibition to bacterial adherence and antibacterial activities. The primary aim of this study was to evaluate the antibacterial activities of pure metals, including selenium, germanium and...
March 2014
Interaction between propoleum extracts and Ciprofloxacin and Levofloxacin for the in vitro inhibition of methicillin-resistant Staphylococcus aureus isolates
To combat Staphylococcus aureus-associated infections, mainly in the respiratory tract, part of the population commonly use or combine “natural” remedies with drugs that are prescribed by health professionals, thinking that in this way, a greater effect will be exerted on the infection. The objective of the present work was to evaluate the in vitro effect of two extracts of propoleum (or propolis) (PPL) on...
March 2014
Polymerase chain reaction (PCR4)-based detection of hly and plc-A genes in Listeria monocytogenes isolated from dairy and meat products in Iran
Listeria monocytogenes is a ubiquitous Gram positive organism causative agent of many hazardous conditions like meningitis and sepsis. To isolate L. monocytogenes from various food samples collected from different markets in Iran and evaluate pathogenic potential of the isolates by determining two virulence associated genes (hly and plc-A) and sequencing them, a total of 130 samples including dairy and meat products...
March 2014
Changes in tRNA profiles in Sinorhizobium meliloti CCBAU 81024 during alkali-shock
The changes of tRNA under alkali shock by the method of stable low-molecular-weight RNA profiles was firstly reported. In Sinorhizobium meliloti CCBAU 81024, there are a clear difference profiles obtained from cells exposed to alkaline shock. In contrast, the profiles from cells under alkaline stress conditions are undistinguishable from cells cultured at pH 7. It was shown that tRNA(s) play some roles in response to...
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