July 2014
Phytochemical profile and antioxidant and antimicrobial activities of hydroethanolic extracts of Ficus pumila
Natural products, mainly of plant origin are widely used in popular medicine. This study aimed to evaluate the therapeutic potential by examining the hydroethanolic extracts of fresh and dried leaf, stem, root and fruit of Ficus pumila concerning their phytochemistry profile and antimicrobial, antioxidant and cytotoxic activities. The results show the presence of tannins and flavonoids in all extracts. The dried root...
July 2014
Microbiological and physicochemical assessments of groundwater quality at Punjab, Pakistan
The assessment of groundwater is essential for the estimation of suitability of water for safe use. An attempt has been made to study the groundwater at the district level of Punjab, Pakistan. These samples were analyzed for various water quality parameters like pH, color, odor, conductance, total suspended solids, trace metals (Fe, cu, B, Ba, Al, Cr, Cd, Ni, Mn and Se), ionic concentration (HCO3, CO3, Cl, SO4, Na, K,...
July 2014
Expression of two loopsV3 from HIV-1 fused to cholera toxin A2B subunit using Lactococcus lactis as a vector to induce immunity in mucosa
Lactococcus lactis, a nonpathogenic bacterium has been used widely as a vector to deliver antigens to the mucosa; one of the principal threats to world health is AIDS and HIV infection, involving the passage of the virus across a mucosal surface. We used L. lactis to express V3 loop from HIV-1, A2B subunit of cholera toxin and their fusion with one or two loops V3 (V3A2B and V3V3A2B). Six-weeks-old Balb/c mice were...
July 2014
Comparative analysis of anti-Staphylococcus aureus action of leaves and fruits of Anadenanthera colubrina var. cebil (Griseb.) Altschul
This study evaluates the antimicrobial activity against Staphylococcus aureus and phytochemical constituents of leaves and fruits of Anadenanthera colubrina var. cebil. the hemolytic action of active fractions was also checked. After a liquid-liquid partition of hydroalcoholic extracts, the following fractions were obtained: cyclohexane (CFL), ethyl acetate (EAFL), n-butanol (NBFL) and aqueous fractions (AFL) from...
July 2014
Pathogenicity of monosporic and polysporic Bipolaris sorokiniana isolates to wheat seed and seedling under controlled conditions
Bipolaris sorokiniana may present considerable genetic diversity and highly variable pathogenicity and virulence. The pathogenicity of 99 B. sorokiniana isolates (27 polysporic and 72 monosporic isolates) from Brazil and other countries was assessed. Based on aborted germination, black point of seed, leaf spot, and coleoptile lesion, the principal component analysis (PCA) was used to evaluate the similarity patterns...
July 2014
Correlation between inoculum volume, positivity rates and microorganisms isolated from blood cultures
The study aimed to assess the volume of blood inoculum in each culture bottle and its effect on the rate and time to positivity, types of microorganisms isolated and contamination rate. During a period of six months, two sets of blood cultures (aerobic and anaerobic: 3006 bottles each) were collected. Of the bottles collected, only 2916 aerobic and 2898 anaerobic culture bottles were analysed by categorising the samples...
July 2014
Bioefficacy of Trichoderma isolates against soil-borne pathogens
The study of morphology and bioefficacy of Trichoderma was undertaken to select the effective isolates against soil-borne pathogens. Fifty one (51) isolates (23 isolates of Trichoderma virens and 28 isolates of Trichoderma harzianum) were morphologically characterised based on the growth characteristics on PDA medium, the size and shape of phialides and conidia.These isolates were screened for bioefficacy against soil...
July 2014
Influence of white-rot fungi on chemical composition and in vitro digestibility of lignocellulosic agro-industrial residues
In this work, we tested the ability of white-rot fungi to alter the chemical composition of lignocellulosic agro-industrial residues so that they could be more susceptible to microbial degradation by rumen microorganisms, thus improving in vitro digestibility of neutral detergent fiber. Agro-industrial residues that were incubated for 60 days with Pleurotus ostreatus PLO06 or Lentinula edodes UFV73 showed significant...
July 2014
Arbuscular mycorrhizal fungi diversity in revegetated areas after bauxite mining
Arbuscular mycorrhizal fungi (AMF) are obligatory biotrophs that have a symbiotic evolutionary relationship with about 80% of all terrestrial plant species. The fungus mainly supplies water and nutrients to the plant and receives photoassimilates. The AMF diversity affects both the competition among species and floristic composition of an area. Fluctuations in the population of this group of microorganisms can cause...
July 2014
Isolation and characterization of Escherichia coli O91:H21 in a sample obtained from cattle
The Escherichia coli Shiga toxin (Stx) producing strains may cause medical conditions that range from diarrhea to hemolytic uremic syndrome; life-threatening conditions worldwide. Shiga toxin-producing E. coli (STEC) strains isolated from patients who usually possess, in addition to one or more stx genes, the eae gene, encoding adhesin intimin. However, a subset of STEC strains associated with human disease lack eae....
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