African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Bioefficacy of Trichoderma isolates against soil-borne pathogens

N. Srinivasa
  • N. Srinivasa
  • Division of Plant Pathology, Indian Agricultural Research Institute (IARI), Pusa campus, New Delhi-110012, India
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T. Prameela Devi
  • T. Prameela Devi
  • Division of Plant Pathology, Indian Agricultural Research Institute (IARI), Pusa campus, New Delhi-110012, India
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S. Sudhirkumar*
  • S. Sudhirkumar*
  • Department of Agricultural Entomology, UAS, GKVK, Bengaluru-560 065, Karnataka, India
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Deeba Kamil
  • Deeba Kamil
  • Division of Plant Pathology, Indian Agricultural Research Institute (IARI), Pusa campus, New Delhi-110012, India
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Jyoti Lekha Borah
  • Jyoti Lekha Borah
  • Division of Plant Pathology, Indian Agricultural Research Institute (IARI), Pusa campus, New Delhi-110012, India
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N. Prabhakaran
  • N. Prabhakaran
  • Division of Plant Pathology, Indian Agricultural Research Institute (IARI), Pusa campus, New Delhi-110012, India
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  •  Received: 24 December 2013
  •  Accepted: 26 May 2014
  •  Published: 09 July 2014

Abstract

The study of morphology and bioefficacy of Trichoderma was undertaken to select the effective isolates against soil-borne pathogens. Fifty one (51) isolates (23 isolates of Trichoderma virens and 28 isolates of Trichoderma harzianum) were morphologically characterised based on the growth characteristics on PDA medium, the size and shape of phialides and conidia.These isolates were screened for bioefficacy against soil borne plant pathogens (Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii) based on their percent inhibition observed during dual culture, volatile and non-volatile methods. Eight T. virens isolates and 12 T. harzianum isolates were proven to be potential isolates against the soil-borne pathogens tested. No correlation was found between bioefficacy and morphology in both species isolates.

Key words: Trichoderma virens, Trichoderma harzianum, morphology, dual culture, biocontrol and bioefficacy.