African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 357

Full Length Research Paper

Extraction, purification and nuclear magnetic resonance (NMR) assignment of garcinoic acid from Garcinia kola (bitter kola) fruit

B. Jones Orock
  • B. Jones Orock
  • Centre for Synthesis and Chemical Biology, School of Chemistry and Chemical Biology, University College Dublin, Dublin 4, Ireland.
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J. Henry Blackwell
  • J. Henry Blackwell
  • Centre for Synthesis and Chemical Biology, School of Chemistry and Chemical Biology, University College Dublin, Dublin 4, Ireland.
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Yannick Ortin
  • Yannick Ortin
  • Centre for Synthesis and Chemical Biology, School of Chemistry and Chemical Biology, University College Dublin, Dublin 4, Ireland.
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Paul Evans
  • Paul Evans
  • Centre for Synthesis and Chemical Biology, School of Chemistry and Chemical Biology, University College Dublin, Dublin 4, Ireland.
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  •  Received: 22 February 2018
  •  Accepted: 26 April 2018
  •  Published: 31 May 2018

Abstract

Continuous extraction of bitter kola nuts, followed by liquid-liquid extraction and flash column chromatography leads to the isolation of garcinoic acid (-tocotrienoloic acid), as the main lipophilic component in a 0.8% yield of the initial dry weight. This compound, an oxidised member of the vitamin E family, was structurally characterised using nuclear magnetic resonance spectroscopy.

Key words: Vitamin E, tocopherol, antioxidant, two-dimensional nuclear magnetic resonance spectroscopy.