African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2134

Full Length Research Paper

Assessment of antibiotics production potentials in four actinomycetes isolated from aquatic environments of the Eastern Cape Province of South Africa

Cwala Z., Igbinosa E. O. and Okoh A. I*.
Applied and Environmental Microbiology Research Group (AEMREG), Department of Bio-chemistry and Microbiology, University of Fort Hare, Alice 5700, Private Bag X1314, South Africa.
Email: [email protected]

  •  Accepted: 21 January 2011
  •  Published: 28 February 2011

Abstract

Crude extracts of four actinomycetes species belonging to Actinoplane [NB 034],Streptomyces [TR 007] and Actinopolyspora [NB 069 and TR 008] genera were screened for antibacterial activities against a wide range of bacterial strains. The extracts showed antibacterial activities against both Gram-negative and Gram-positive test bacteria with zones of inhibition ranging between 2 and 27 mm (NB 034); 9 and 15 mm (TR 007); 8 and13 (NB 069) and 2 and 12 mm (NB 069). The minimum inhibitory concentration (MIC) of the extracts against the test bacteria ranged from 0.039 - 5 mg/ml, while the minimum bactericidal concentrations (MBC) ranged from 5 to >10 mg/ml. The extracts exhibited concentration-time dependent killing of Staphylococcus aureus OKOH2A and Staphylococcus scuiri OKOH2B with a 0.73log10 and 0.88log10reduction in viable counts respectively, while a re-growth phenomenon was exhibited byKlebsiella pneumonia ATCC 4352 and Escherichia coli ATCC 25922 at MIC, 2 × MIC and 4 × MIC of Streptomyces (TR 007) Actinopolyspora (TR 008) extracts. All the active extracts exhibited bacteriostatic activities.

 

Key words: Actinoplane, Streptomyces, Actinopolyspora, antibiotic production.

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