African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2201

Full Length Research Paper

Levels of red blood cells derived microparticles in stored erythrocyte concentrate

Maheen Rana
  • Maheen Rana
  • Department of Pathology, Rashid Latif Medical and Dental College, Lahore, Pakistan
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Yasir Arfat
  • Yasir Arfat
  • College of Bioresources Chemical and Material Engineering, Shaanxi University of Science and Technology, Xian, China.
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Omar Naseem
  • Omar Naseem
  • Department of Pathology, Abu Ammara Medical College, Lahore, Pakistan.
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Nazish Mazari
  • Nazish Mazari
  • Department of Pathology, Al-Nafees Medical College Isra University, Islamabad, Pakistan.
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Navida Manzoor
  • Navida Manzoor
  • Department of Pharmacology, Rashid Latif Medical and Dental College, Lahore, Pakistan.
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Rao Salman Aziz
  • Rao Salman Aziz
  • Department of Pharmacology, Rashid Latif Medical and Dental College, Lahore, Pakistan.
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Muhammad Rashid
  • Muhammad Rashid
  • Faculty of Fisheries and Wildlife, University of Veterinary and Animal Sciences, Lahore, Pakistan.
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Shahida Mohsin
  • Shahida Mohsin
  • Department of Haematology, University of Health Sciences, Lahore, Pakistan.
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  •  Received: 19 February 2020
  •  Accepted: 16 April 2020
  •  Published: 31 July 2020

Abstract

There is increasing evidence of the clinical importance of microparticles (MPs) and their role in blood transfusion-related side effects and the transmission of pathogens. The study aims to examine the red blood cell-derived MPs in blood bags during storage under standardized blood bank conditions. The samples were tested at various times to demonstrate the presence of RBC-derived MPs by flow cytometry. The quantitative assay was carried out in stored erythrocyte concentrate on days 0, 25 and 35 and their number from day 0 to 25 and 35 and the number of day 25 to day 35 were compared. The MPs were counted after being concentrated in a supernatant (labeled with the respective antibodies CD47, CD235a and Annexin V) obtained by a specific centrifugation procedure. The analysis showed that the number of Annexin V positive MPs increased between day 0 and day 35 (~ 0.001) and CD47 expression on MPs at day 25 and day 35 decreased compared to day 0 (~ 0.001). In addition, CD235a expression had shown minimal insignificant changes with an upward trend (> 0.05) during the storage period. It is concluded that monitoring the release of MPs from RBC units during storage is a sensitive approach to identifying MPs for transfusion drugs and, more broadly, for cell-based therapies.

 

Key words: Red blood cells, phosphatidylserine, cell-derived microparticle.