African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2201

Full Length Research Paper

Expression and significance of glyceraldehyde-3-phosphate dehydrogenase in patients with osteosarcoma

Dan Fei1, Hejia Zhang1, Zhongli Gao2, Dan Jiao1, Guoqing Sui1 and Kaijun Zhao1*
1Department of Ultrasonographic, China-Japan Union Hospital of Jilin University, Chang Chun130033, China. 2China-Japan Union Hospital of Jilin University, Chang Chun130033, China.
Email: [email protected]

  •  Accepted: 24 September 2012
  •  Published: 31 October 2012

Abstract

To observe the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in patients with osteosarcoma and its relationship with surgical stage, 60 patients with osteosarcoma, including 20 cases in Stage I, 17 cases in Stage II and 23 cases in Stage III were sequentially enrolled in this study. The expression of GAPDH in osteosarcoma and osteochondroma tissues on mRNA and protein levels were measured by fluorescent quantitative polymerase chain reaction (qPCR) and immunohistochemical stain, respectively. It was found that GAPDH was significantly up-regulated in osteosarcoma in comparison to the healthy control on mRNA and protein levels (P < 0. 05). The positive rate of GAPDH osteosarcomas in Stage III were significantly higher than those in Stages I and II (P < 0. 05). These data demonstrated that the expression of GAPDH correlates with the clinical stage of osteosarcomas, which implied that over-expressions of GAPDH play an important role in the development of osteosarcoma.

 

Key words: Osteosarcoma, GAPDH, qPCR, Immunohistochemical stain